We narrowed to 402 results for: LARGE;
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TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....you want to use in a given experiment. For very large panels (upwards of 30-40 colors), spectral flow ...particularly useful tool for those wishing to run larger panels: five laser spectral flow machines have ...fluorophores. This allows for the creation of much larger panels, as fluorophores that cannot be distinguished...
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Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....at two sites within the viral genome to excise a large portion of it. This ongoing trial is sponsored by... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m.... and time-consuming to generate, especially for large animals such as primates. Viral vectors with cell-type... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....cells with unique spatiotemporal phenotypes from large heterogeneous cultures. HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....the conference you are attending on the larger side? At large conferences, chances are that several talks... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....could be good choices if you have a particularly large number of inserts to assemble. You could also consider... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....indicating that Met protein functions have been largely conserved since the species diverged. Tips on using... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m.... commonly observed with direct stimulation are largely absent in the soCoChR-expressing system. Taken ... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....Steitz was presented with a physical model of his large ribosomal subunit, he kept sliding his palm across...the models we create are around 30 million times larger than the real thing. Nevertheless, the models are... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....the possibilities can seem overwhelming given the large assortment of fluorescent proteins available. Fortunately... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m.[email protected]. These deposits are considered large deposits and are exceptions to our standard workflow... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and... -
Technical Design of a Western Blot
TypeBlog PostPublishedAug. 22, 2024, 1:15 p.m....Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm). Types of transfers...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m.... prone to recombination, while a library with a large backbone might be more difficult to transform. ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m..... Picking receptors The receptor you choose is largely dependent on what cellular response you aim to ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....Permeabilization What is the point? Antibodies are large proteins that need a little help crossing cell membranes...