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We narrowed to 473 results for: NOL

Showing: 221 - 240 of 473 results

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules...effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new...

  3. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips (USA...where it immediately spreads out to form a firm monolayer. The cell clones - easily identified from the ...

  5. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from...Scientists are making progress. New resources and technologies become available almost daily, yet obstacles...

  7. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed PMID: 25184501. PubMed... off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184. PubMed PMID: 26780180. PubMed...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic...research is being done to further improve the technology: engineering of new capsid variants, designing...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...anchoring strategy for expansion microscopy. Nat Biotechnol 1–12. https://doi.org/10.1038/s41587-022-01546...standard fluorescent proteins and antibodies. Nat Biotechnol 34:987–992. https://doi.org/10.1038/nbt.3625  ...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...RNase to remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins...of four times before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein...High Throughput Cloning Strategies Method Technology Pros Cons References Golden Gate Type II restriction...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts... many other uses currently exist, and new GFP technology is constantly being developed! Fusion tagging...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...

  16. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... my work. The meetings often are the work. Will Arnold, one of the newer members of our Scientist team...and for modeling in the picture above), and Will Arnold for their ideas and tips! Additional resources ...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett syndrome mutations...three neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... be used in some in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  ... heterologous protein expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...adeno-associated viral (AAV) vector toolbox. Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002...in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102–106 . https://doi.org/10.1038/nbt.3055...
Showing: 221 - 240 of 473 results