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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 628 results for: Ncl

Showing: 221 - 240 of 628 results

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression...

  3. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Statistics (OES), but international Twitter users were included in the dataset; 2) users not part of a scientific...scientists who follow each other, or are friends, are included in this cluster analysis. The closer two dots ...

  4. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images...

  5. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ...

  7. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...

  8. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and neurotransmission... could be measured using the TRUPATH system. Conclusions Although the importance of GPCR pathways is well...

  9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a limiting...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable ...

  10. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...members of the ubiquitous activin ligand family, including Activin A. A commercially developed therapeutic...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...

  13. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,...

  14. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

  15. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...are some highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, ...sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and ...

  20. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...standardized methods for evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise...guest bloggers Dominik Paquet and Dylan Kwart, including a detailed look at how cut-to-mutation distance...
Showing: 221 - 240 of 628 results