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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 459 results for: PAC;

Showing: 221 - 240 of 459 results

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...distributed milestone! We were thrilled to see the impact our repository was having on the scientific community...expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

  5. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...Future of Research Learn How Notions of Scientific Impact Are Evoloving Check Out Extracurricular Activities...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...the many freely-available DNA-editing software packages to assemble your expected full plasmid sequences...when designing a sequencing primer, you need some space between your primer binding site and the first feature...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...

  8. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...both technically challenging and can negatively impact zygote viability. Nanoblades, however, avoid this...this pitfall since they are injected into the space surrounding the zygote, where they can then fuse with...

  9. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

  10. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ...the calcium sensors described are also already packaged into viral vectors and ready to go. Pro tip: Vectors...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

  12. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection....   Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.  ...

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...

  14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...

  15. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

  16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...first keynote presentation. He talked about the impact of the Human Genome project on its 25th anniversary... how the genome project has already had a major impact on undiagnosed and rare diseases; Before the Human...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that...

  18. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...a team to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed...better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...that is complementary to the target site, or protospacer, in the genome. Native Cas9 has two catalytic...which cleaves one strand of DNA upon binding the protospacer. The resulting double strand break (DSB) stimulates...

  20. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...
Showing: 221 - 240 of 459 results