We narrowed to 461 results for: PAC;

...amazing affinity reagents we just didn't have the space to give proper credit to. Thankfully, as Addgene's..., or be able to access an epitope folded into a space that would size-exclude an antibody. In imaging ...

...therapy delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...

...distributed milestone! We were thrilled to see the impact our repository was having on the scientific community...expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

...different directions is sometimes at odds with the space needed to be creative. “You need a lot of time to...readers with it.” She feels that storytelling is an impactful tool for science communicators. “The power of ...

...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

...enough workshops by herself to make the biggest impact. When she spoke with Teytelman about an opportunity...Use your network well to seek out valuable and impactful opportunities - There are many brilliant minds...

...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...Future of Research Learn How Notions of Scientific Impact Are Evoloving Check Out Extracurricular Activities...

...the many freely-available DNA-editing software packages to assemble your expected full plasmid sequences...when designing a sequencing primer, you need some space between your primer binding site and the first feature...

...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...

...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ...the calcium sensors described are also already packaged into viral vectors and ready to go. Pro tip: Vectors...

...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection....   Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.  ...

...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...

...breed. Some problems with transgenic mice that can impact reproduction include embryonic lethality, a short...should be tested in cell lines to determine how it impacts the expression level of the transgene. In existing...

...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

...both technically challenging and can negatively impact zygote viability. Nanoblades, however, avoid this...this pitfall since they are injected into the space surrounding the zygote, where they can then fuse with...

...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...

...first keynote presentation. He talked about the impact of the Human Genome project on its 25th anniversary... how the genome project has already had a major impact on undiagnosed and rare diseases; Before the Human...

...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus lugdunensis that...

...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...