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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 485 results for: Ret;

Showing: 221 - 240 of 485 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...researchers realized they needed specific tools to interpret the roles of individual integrins. The Ha lab ...

  2. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...This means all newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in...invasive strand is displaced from the template and returned to the processed end of the non-invading strand...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...composition.  So back to the drawing board.  Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...good solution. The process of error-prone PCR is pretty much exactly what it sounds like — a PCR, but with...antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and more coming...

  9. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no....

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR...editors (calling them GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing...

  15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    .... Other techniques, like electron microscopy and FRET, can offer more information on these regions.   ...SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., 2022). ...

  17. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in a single well.... Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. ...

  18. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12/2014). With so many options...g3.112.005439  Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...has both high binding capacity and high protein retention. It comes in a variety of pore sizes and protein...with high binding capacity, though it has lower retention after binding and washes. It’s less sturdy and...

  20. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... rule of thumb, the more strictly you want to interpret your blot’s data, the more time you’ll want to...starting a western blot, think about how you’ll interpret the data and what level of accuracy you’ll need...
Showing: 221 - 240 of 485 results