We narrowed to 251 results for: SPI;

1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...only saw incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our...

2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...wheat, mushroom, and rice (Khatodia et al., 2016). Despite the almost universal functionality of the CRISPR...

3. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   Published

   June 23, 2016, 2:30 p.m.

   ...organisms lacked a classic cysteinyl tRNA synthetase, despite abundant incorporation of cysteine in their proteomes...

4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...strategy may be a better option than direct cleavage. Despite the potential difficulties in translating these...

5. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   Published

   Jan. 26, 2016, 3:30 p.m.

   ...wasting leading to death at around 30 years of age. Despite the amount of research conducted on DMD, there ...

6. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

7. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves...

8. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   Published

   Oct. 2, 2018, 12:56 p.m.

   ...Will you learn how to run a lab if that is your aspiration? Will you help or at least observe grant writing...

9. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   Published

   July 28, 2016, 2:30 p.m.

   ...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...

10. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...

11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ...cardiac, and retinal cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied...

12. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    Published

    Dec. 20, 2022, 2:15 p.m.

    ...to the promoter and turning transcription OFF. Despite their bacterial origins, Tet systems function well...

13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...Boyden et al., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history ...

14. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...these containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers...

15. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ...advancements and the noteworthy work of fiction that it inspired. CAUTION: Spoilers alert! Pre-DNA Era  H.G. ...

16. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ...new pieces of data, your talk will probably not inspire you or the audience. Ask yourself: What is the...

17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...

18. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...

19. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...to their Biosafety Level-2 or 2+. Please Note: Despite the fact that AAVs themselves are not pathogenic...

20. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...order to aid viral binding. This method, called spinoculation, has been found very effective for T cells transduction...