We narrowed to 859 results for: STI;

...Daley, Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding...particularly in fields like stem cell biology where questions of ethics can have profound impacts on the development...banked iPSCs across international borders. While questions of ethics can be daunting because they have no...cell community for addressing these important questions that the scientific method cannot address. Listen...already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing...

...repair bias is a fascinating (and yet unanswered) question. Our recent paper (Richardson et al 2016) revealed...that process. We began our research with a simple question: how do Cas9 and sgRNA interact with and dissociate...from a DNA target? The surprising answer to this question led us to develop a new, rational approach to ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...repair that normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

...life of its own. Nevertheless, there are still many open questions to be clarified, especially with regard... field, often referred to as 'frugal science', existing components are adapted and combined with creativity...raw data (CARE), segmentation of imaging data (ILASTIK), or the popular plugin-based image process framework... put their work on the shoulders of giants. The still relatively young movement of sharing open hardware...References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic microscopy for everyone: the...

...we can’t forget that those other components are still there in real life.  Certainly referring to any ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...consider searching directly on our website. In-exhaustive Chromatin-related genes with plasmids on Addgene.org...@theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene...

... the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...requires more optimization. Enzymatic digestion, while gentler on the sample, introduces bias due to the...sonication is usually the preferred method, enzymatic digestion can be appropriate when your protein of interest...cofactor. In such cases, the gentler enzymatic digestion can preserve more of the protein:DNA complexes...After crosslinking and sonication or enzymatic digestion, you’re ready to incubate the sample with your...

...Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected ...site-directed mutagenesis experiment? The answer to both questions is the same--methylation! Read on to learn about...from the restriction modification systems, yet can still affect whether certain restriction enzymes can effectively...potential for methylation should be considered when digesting DNA. Why? Well, even though Dam methylation sites...performing a digest for cloning purposes or for diagnostics, we suggest double checking to make sure your...affected by methylation. This table is by no means exhaustive, so you may want to consult the REBASE database...

...toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs...any 5’ leading nucleotide could be processed, a distinct advantage over other methods that favor certain...indicating that Cas9 can be guided to at least 8 distinct loci. They also examined editing efficiency in...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression strategies...systems available from Addgene. If you have any questions or thoughts about this technology, leave them ...them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability with the endogenous...

...synthetic biology is still in its infancy. A long timeline from transformation to testing and a lack of well-characterized... of plants are being investigated on limited funding and time. While institutions that focus on creative...problem solving like the Howard Hughes Medical Institute have begun to pivot towards plant biotechnology...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...

...varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic nature. The potency...microscopy techniques, such as Stimulated Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy...help. Here, we will outline some fundamental characteristics to consider when selecting a dye or ligand ...Fluorescence microscopy takes advantage of the characteristics of these fluorophores to enable highly specific...non-fluorescent states in response to a specific stimuli, which leaves only a small volume of fluorophores...

...appropriate containers. The machine is useful for distinguishing cell populations that have been tagged with...visualize how cells interact after a particular stimulus. Using an epifluorescence microscope and GFP fusion...easily track GFP expressing cells. For example, by harvesting lymph nodes at different times after antigen ...immunologists the ability to count cells and statistically analyze cell movements. By using cells labelled...immune response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able...aureus and eating the bacteria. Some of the many questions scientists have been able to help answer using...understood thanks to these technologies but many questions remain. The use of other technologies such as ...

...stranded RNA (ssRNA), while some exist as dsRNA. Still others use dsDNA to store their genetic information... made it into agriculture as species-specific pesticides and insecticides, an advancement over previous...sequence. All of the R-loops described above play distinct roles in promoting cellular processes and functions...While not perfect, these sensing systems work to distinguish between RNA of cellular vs. viral origin and ... each one acting on specific substrates and in distinct cell cycles. When RNA:DNA hybrids levels are allowed...appreciate double stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses...

... gene expression. On the other hand, using a constitutive promoter in the same background resulted in ...over-represented compared to those further away. While constitutive promoters drove maximal gene expression near...during shifts in growth rate as compared to a constitutive promoter. To simulate the increase in plasmid...When they increased concentrations of IPTG, a constitutive promoter upstream of GFP increased expression...stabilized promoter while gene expression from the constitutive promoter did not. Similar levels of expression... al. placed the genes under the control of a constitutive promoter and then moved them into the genome...

... of interest in living cells. Challenges with existing RNA-protein interaction methods Not in a native...time point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli...based proximity labeling methods, RaPID cannot distinguish between proteins that directly or indirectly ...sounds like a nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline ...of QKI decreased Zika viral RNA levels by 90%, suggesting that QKI is a host protein critical to Zika replication... study RNA-protein interactions. Do you have a question that RaPID could help answer? The RaPID plasmids...

...science - which can drive away even the most enthusiastic of learners.  What contributes to cognitive ...information and a logic to the types and timing of questions that are asked. Many things, therefore, will be...connection for your audience. I ask myself that question constantly (and so should you!)  Amount of information...as valuable feedback.  Listen. Think about the questions you’ve received, read the comments, seek feedback...  So now that you have all this knowledge, the question is: How do you know what’s too much (or too little...planned; other times students were fired up and enthusiastic and needed a faster presentation with more information...

...To LinkedIn or not to LinkedIn. That is the question. When presenting on building relationships (also...known as “networking”), one of the most common questions I receive is “Do I have to be on LinkedIn?” For...information available. When I am speaking at a new institution, for example, I will often see a whole bunch ...rule, but not that many). I’m asked a lot about requesting connections to complete strangers for informational...companies and organizations to see their news and job postings. 4) Use LinkedIn to pay it forward The best way... manager. This is considered intrusive and it’s still connecting with a stranger so is highly discouraged...

...participating in science and engineering. “Fix the Institutions” promotes gender equality in careers through...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating...study saved lives. Reduced use of estrogen plus progestin hormone therapy resulted in 76,000 fewer cases...the importance of analyzing sex, the National Institutes of Health (NIH) now requires that sex be included...Using cells from both sexes ensures that the diagnostics and therapeutic interventions you develop represent... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...

...regulation thats as sophisticated as a natural network. We could build a material as sophisticated as wood. To...Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on new experimental...to work with than regulatory proteins. There are still things we haven’t solved that stop it from being... carbons and oxygens. Nothing too much more sophisticated. There are also proteins, like antibodies. But...able to design new materials and chemicals as sophisticated as those that living cells and animals make....

...cycle once inside the host. While the virus can still enter (and thus infect) cells, this safety feature...Separation of retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific...cabinet and decontaminate after use. Virus-exposed plastics and other surfaces should be thoroughly decontaminated...decontaminated after use, typically with bleach (plastic) or ethanol (other surfaces). For AAVs, which are...should be added. After decontamination, single-use plastics and other handling tools should then be disposed... to reduce the risk of exposing yourself via needlestick or cut. Following all sharps procedures for your...

...past, we've been working with the Rare Genomics Institute to provide plasmid grants to researchers working...mechanisms behind the immune defects in CDG patients are still poorly studied and understood.  In line with this...complexes, but a serendipitous discovery led them to investigate the use of a common laboratory chemical, guanidine...Engineering the human disease alleles into cells for testing in the lab will be greatly facilitated by CRISPR...sites in T4 lysozyme by deficiency-creating substitutions." Journal of molecular biology 277.2 (1998):...significance (VUSs) that are frequently found in genetic testing for syndromes that include developmental delay,...

...other species, but these studies generally leave questions about gene function wide open. What if two seemingly...over time? Undergraduates can help to answer this question! The Pathways Over Time project The Pathways ... a framework for these undergraduate research questions. The idea is straightforward. Instructors first...have been using yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine...experiments that are used to answer our research question, the plasmids are also used to teach students ...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...