... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

...like the cold or flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics...those bacteria that are partially resistant. If you stop a treatment early, you may be un-wittingly selecting...used in the clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  The problem...

...a room, but still have control over focus, zoom, stop/start record, and allow me to see an image.  Remote...control some basic functions, including starting and stopping recording).  Test: It was unbelievable how well...the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need...

...modification with fatty acyl chains. For instance, myristoylation requires an N-terminal consensus sequence (...variants and check the functionality of the fusion (Mastop, 2018). Summary GFP is an all-round useful genetically...21619590. PubMed Central PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring...

...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...

...at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...integrin antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim...

... in your specific experimental context (Pillai-Kastoori et al., 2019). Tissues or cell lines other than...and references References Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory...://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache ...

...the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant female mice...s a guide that directs cleavage right before the stop codon!  Figure 3: How to design a C-terminal...guide would be selected, since it cleaves at the stop codon TAG. Design your repair template with ...

...and whose structures are just beginning to be understood. Therefore, in addition to their utility in the... strain that you can use for long-term library storage. After overnight growth, scrape up, pool and extract...during one of these steps is also a convenient stopping (ie. going home and eating and sleeping) point...

...2006): 703-9. PubMed PMID: 16582903. Potter, Christopher J., et al. “The Q system: a repressible binary...from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling...s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a functional...

...adapt. By the end of my three months, I fully understood why parasitology roles in the field require experience... available at a small science non-profit doing customer service (recruitment agencies often don’t tell...I was also surprised to learn that I enjoy the customer service side of my job. Because of my background...

...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....mean it’s a bad antibody but more that it’s misunderstood and it could work great in another context! ...

... viruses were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into ...gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches...enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ...

...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...performing quality control, and providing technical customer service. Team members are highly trained, and ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

...Despite its relatively recent development, the history of optogenetics begins almost 50 years ago with...Archaea, the third in green algae (Oesterhelt and Stoeckenius, 1971; Matsuno-Yagi and Mukohata, 1977; Foster...PMID: 22480664. Oesterhelt, Dieter, and Walther Stoeckenius. "Rhodopsin-like protein from the purple membrane...

...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...bind to and inhibit the function of a protein to stop its effect within the body, or they can be used ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

...workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein isolation, then the ...more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably realize ...et al. “A Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol...

...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

...leads to the first base transversion editor The story of base transversion editors starts with researchers...the SOX2 activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing... newly synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post...