We narrowed to 497 results for: SUB;

... when they begin their own experiments. Need to submit an updated protocol? Just email us at [email protected] and protocol management. Though this is a subscription-based software, Labguru does offer free trials...

...checkbox and paste your reference sequence into the Subject Sequence box that appears. Aligning two nucleotide...pasting your expected protein sequence into the Subject Sequence box. BLAST alternatives Depending on your...

...precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues in ... on the ADAR2DD residues that contact the RNA substrate resulted in 15% C-to-U editing activity. They ...

..., is an exercise in restraint. The very first subheading in Chapter 1 asks “What is science?” That’s a...over the coming years. It is arguably the most subjective chapter of the book, but that’s unlikely to be...

...detergents have absorbance maxima around 230 nm and subsequently lower the A260/230 ratio of a sample. Ethanol... concentrations. However, such systems are no substitute for sound techniques, routine machine maintenance...

... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

... the last step is to mini-prep these clones and submit the plasmids for Sanger sequencing. Sequencing ...there aren’t mutations in your insert. Make sure to submit multiple positive clones for sequencing to verify...

...range from coordinating the grant application and submitting it, to evaluating the capacity of an organization...deadlines, and we ensure that grant reports are submitted and project evaluations are completed. To enter...

...by, for example, helping to improve the papers submitted to journals. In addition, it is my pleasure to...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. ...

...interchangeable. While these differences may seem subtle they result in a key physiological difference between...useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted...

... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...

...directly accomplish the base edit or to create the substrate for a base excision repair pathway. The team behind...UNG enzyme was modified to recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited...

...multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova... Tag catalyze a luminescent reaction with the substrate furimazine. Right: Demonstration of PILATeS. Cell...

.../dev team rarely, if ever, sees cover letters submitted for their entry-level jobs and does not require...there were examples of great hires who hadn’t submitted a cover letter; their experience and skills had...

...Editing of these motifs generates lesions that are subject to excision and ssDNA cleavage by canonical base... provides a read out for plasmid delivery and subsequent base editing efficiency. TLDR: TREE from the ...

...transcription to convert amplified DNA to RNA for subsequent detection by Cas13. This amplification step in...trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded at a rate of ~1,250 cuts per second...

...market? In addition to all of the documents you submit, you will need to present a "chalk talk." However...mini-organs or organoids, neither of these can substitute for a liver transplant. My lab will combine bioengineered...

...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron, 109(9), 1449-1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

... activating chemogentic receptor (hM3Dq) into a subset of neurons within the LC of mice. When those neurons...further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible...

...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...behavior. Signs of a mycoplasma contamination can be subtle and may include reduced saturation density, decreased...