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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 495 results for: SUB;

Showing: 221 - 240 of 495 results

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...checkbox and paste your reference sequence into the Subject Sequence box that appears. Aligning two nucleotide...pasting your expected protein sequence into the Subject Sequence box. BLAST alternatives Depending on your...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues in ... on the ADAR2DD residues that contact the RNA substrate resulted in 15% C-to-U editing activity. They ...

  5. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ..., is an exercise in restraint. The very first subheading in Chapter 1 asks “What is science?” That’s a...over the coming years. It is arguably the most subjective chapter of the book, but that’s unlikely to be...

  7. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... the last step is to mini-prep these clones and submit the plasmids for Sanger sequencing. Sequencing ...there aren’t mutations in your insert. Make sure to submit multiple positive clones for sequencing to verify...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...range from coordinating the grant application and submitting it, to evaluating the capacity of an organization...deadlines, and we ensure that grant reports are submitted and project evaluations are completed. To enter...

  9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...by, for example, helping to improve the papers submitted to journals. In addition, it is my pleasure to...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. ...

  10. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...interchangeable. While these differences may seem subtle they result in a key physiological difference between...useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... activating chemogentic receptor (hM3Dq) into a subset of neurons within the LC of mice. When those neurons...further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible...

  12. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...market? In addition to all of the documents you submit, you will need to present a "chalk talk." However...mini-organs or organoids, neither of these can substitute for a liver transplant. My lab will combine bioengineered...

  13. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...transcription to convert amplified DNA to RNA for subsequent detection by Cas13. This amplification step in...trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded at a rate of ~1,250 cuts per second...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...multiple myeloma drug mezigdomide and ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova... Tag catalyze a luminescent reaction with the substrate furimazine. Right: Demonstration of PILATeS. Cell...

  17. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    .../dev team rarely, if ever, sees cover letters submitted for their entry-level jobs and does not require...there were examples of great hires who hadn’t submitted a cover letter; their experience and skills had...

  19. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...directly accomplish the base edit or to create the substrate for a base excision repair pathway. The team behind...UNG enzyme was modified to recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...behavior. Signs of a mycoplasma contamination can be subtle and may include reduced saturation density, decreased...
Showing: 221 - 240 of 495 results