Skip to main content
Addgene
Showing: 221 - 240 of 261 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV...

  2. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Library Amplification protocol are useful references. Skim through our Pooled Library page to find other libraries...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    .... UV light exposure is also harmful for eyes and skin so keep the light off when the hood is in use. Wipe...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...with other members of your lab, a lot of the “soft skills” required for teamwork may not be a requirement...

  8. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...ready-to-use lentiviral preparation from Addgene, you can skip the steps above! Cells are then transduced with ...

  9. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...peptides are thought to function by making the ribosome skip the synthesis of a peptide bond at the C-terminus...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...caused by loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...24664759. PubMed Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...

  18. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...observe any mutations at the target site. In a mouse skin colonization model, phagemid treatment decreased...

  19. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...with protein folding HBH HHHHHHAGKA GEGEIPAPLA GTVSKILVKE GDTVKAGQTV LVLEAMKMET EINAPTDGKV EKVLVKERDA VQGGQGLIKI...
Showing: 221 - 240 of 261 results