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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 487 results for: Vit

Showing: 221 - 240 of 487 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through...gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate deletions ...

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications in which...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...

  4. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...depleting a protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing...performance, great depth of degradation, absolute selectivity, broad utility for degrading targets in different...

  5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...necessary and sufficient for certain patterning activities of entire tissue types or even organs. In frogs...activation. Upon activation, exhibits stabilized activity for over 30 minutes and can be deactivated using...

  6. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

  7. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all... and taking on independent projects, you will inevitably develop problem solving skills passively. But...

  8. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...achieve maximal on-target and minimal off-target activities have proven themselves incredibly useful for ...G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... scared to engage with them. Comment on their activities and participate in discussions. Get your voice...Twitter to promote your other science communication activities, e.g. tweet it when you publish a new blog post...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...researchers can explore synaptic integration and connectivity without the constraints of antibody labeling...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...could in theory promote off-target binding. The activity of SpRY was validated in double stranded cleavage...assessment of the PAM compatibility and editing activities of Cas9 variants. Nucleic Acids Res. 2021 Sep...

  14. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...provides a noninvasive method for manipulating cell activity, especially neuronal firing. What is chemogenetics...Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...important implications for the fusion protein effectivity), and a fluorescent protein tag for visualization...VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...polyadenylation generally serves to promote transcript longevity in eukaryotes and promote transcript degradation...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...

  18. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... Addgene has tested these capsid plasmids in vitro and uses them in our own AAV production processes...for neuronal tracing. Labeling was nontoxic and activity-dependent, with more labeling across active synapses...

  19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection of synthetic...selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each mRNA molecule...

  20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...excess energy at wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths...non-specific binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination...
Showing: 221 - 240 of 487 results