...latter would likely be more effective, so long as care is taken in the interpretation of results by requiring...use of CRISPR technology will always depend on careful experimental design, execution, and analysis. ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate... B., Fong, D. M., Mouravlev, A., Young, D., & O’Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy....2014.00076 Nieuwenhuis, B., Haenzi, B., Hilton, S., Carnicer-Lombarte, A., Hobo, B., Verhaagen, J., & Fawcett...

...al., 2009), and at high copy numbers (Williams, Carnes, and Hodgson, 2009). More research is ongoing to...0092-8674(84)90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design... JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid DNA production platform incorporating...

...health sector, phages represent a reservoir of carbohydrate binding proteins waiting to be explored and ...spot this protein directly onto nitrocellulose and carry out the steps from blocking the membrane to probing...bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion in structural biology 34 (...

...population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to differences in the ...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for... 12. https://doi.org/10.3389/fnsyn.2020.00006 O’Carroll SJ, Cook WH, Young D (2021) AAV Targeting of Glial...

...lesser than the mean of a control group. If you only care if one group is different in a certain direction...rate by only performing the comparisons you really care about. Family-wise error is the probability of a...Groups with Ordinal Dependent Variables: A Monte Carlo Simulation Study. Front. Appl. Math. Stat. Volume...

... compartments in in vivo model systems, so extra care must be taken to assess if proper staining is achieved... such as conjugation with proteins, should be carefully managed. Proper dye-to-protein ratios, or the ... and quenching, which can be mitigated through careful selection and optimization. Fluorescent experiment...

... interpreting your data therefore necessitates careful design of RNAi sequences, confirmation of RNA knockdown...doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm...., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., &...

...Vectors Available at Addgene: Lab Plasmids Dan Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis...target cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of efficiently...there are some drawbacks with this technique. Great care needs to be taken with common S288c strains since...apicomplexan genes remain uncharacterized. Recent studies carried out by Sebastian Lourido and colleagues constitute...

...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

...Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author...Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author: Benedict...

...Chew! Podcast breakdown Intro: Wei Leong Chew's career path and why he chose to go into genome engineering...Chapter 7/Outro: Advice for researchers pursuing careers in academia. Many thanks to our guest, Wei Leong...

...resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry Schaefer, ...reach out to me by email.  About the author Niko McCarty is the editor of Asimov Press and is designing ...

...Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going into academia: follow...

...about how to navigate through all stages of your career, whether you’re a graduate student, post-doctoral...   Caroline LaManna...

...re selected as a winner, you’ll receive a winter care package from Addgene.  Here are the details:   ...  Caroline LaManna...

...sciencedaily #instascience #ladies #research #cardiovascularresearch #parkinsonsdisease A post shared by Ladies...competition. The four winners will receive a holiday care package from Addgene. We hope all of our entrants...

...significant barriers to scientific discovery and career development. They are working with limited resources...with donors to supply surplus lab equipment to carefully-screened research and teaching departments at ...

... advantage of the customizability, choosing the cargo, serotype, and prep size they need for their experiments...Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral ...

...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...