CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
Type
Blog Post
Published
Sept. 28, 2020, noon
...are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI...elements, like a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria...Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7...