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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 535 results for: epo

Showing: 221 - 240 of 535 results

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries in NCBI...NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids!...

  3. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...

  4. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...pathway is ubiquitous, with successful recombination reported in all laboratory E. coli strains tested so far...use of this pathway as a cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck...exploiting bacterial in vivo assembly." Scientific reports 6 (2016): 27459. PubMed PMID: 27264908. PubMed ...

  5. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers...designed a tool that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral...complicating the issue is the long and ugly history of reporting false essentialist links between biology and ancestry...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be...t common for simple transfection experiments. Reporters Finally, in some cases a researcher may choose...

  7. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

  8. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...primary antibody respectively. Avidin bound to a reporter is then added to the reaction for easy detection... experiments. During detection, a biotinylated reporter enzyme that is pre-incubated with free avidin ...protein of interest leading to amplification of the reporter signal and easier detection. This strategy is ...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ... Team is responsible for guiding new deposits into the repository, performing quality control, and providing...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...and were suggested to be the best gRNAs by the depositing lab. The different labs use different algorithms... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... tRNA suppression plasmids are available from depositing labs that have mastered this technique so it ...measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert...protein kinase dynamics in living cells with FRET reporters." Methods 40.3 (2006): 279-286. PubMed PMID: 16908183...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... is not efficient at the protein level at the timepoint studied Off-target activities of the RNAi Correctly... need to study protein expression at multiple timepoints. When the target of interest is critical to cell... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019...

  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared...

  15. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...

  16. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...

  17. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...

  18. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...

  19. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...Milling and Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in ...system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that...

  20. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...
Showing: 221 - 240 of 535 results