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We narrowed to 256 results for: human dat

Showing: 221 - 240 of 256 results

  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... that there is an NGG PAM every 42 bases in the human genome. SpCas9 targeting should be easy, right? ...base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t know ...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...serotype 2-derived inverted terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: ... virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great...hours hands-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi....resistance to both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb...high pH environments and is less susceptible to degradation in agar plates. This prevents satellite colonies...

  4. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...building events like Escape the Room or Habitat for Humanity take you and your co-workers out of the office... up and running repository. As they built the foundation for what Addgene is today, there was a lot of...culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the question...

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015...protein. Such flexibility makes aptamers ideal candidates for biosensors. Aptamer biosensors are used in.... The nature of aptamers makes them excellent candidates for developing assays for otherwise difficult-to-study...

  6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...cytoplasmic environments. However, approximately 40% of human (and most eukaryotic) proteins localize to chemically...suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5]. The moxFPs...of cysteine-free fluorescent proteins for the oxidative environment." PloS one 7.5 (2012): e37551. Pubmed...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... of transcytosis and transduction using primary human brain endothelial cells. J Neurochem 140, 216–230...Aebischer, P. (2010). Efficient transduction of non-human primate motor neurons after intramuscular delivery...endocytosed material or molecules targeted for degradation. These two pathways also use different cytoskeletal...comprised of the viral proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been...

  8. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...between EGFP fragments. The pX330 plasmid contains humanized Cas9 expression cassette and sgRNA expression ...previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure 1: Validation of double... a simple method for selecting the guide RNA, validating its efficacy in vitro, and using it in mouse ... protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette reconstitution...vitro screening is a simple and fast method for validating your selected gRNA’s, and has the bonus benefit...benefit of supplying you with primers for validating your edit in mice once they have been generated (more...more on that later). Find Validated gRNAs to Target Mouse Genes To begin the in vitro screening process...

  9. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like...usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether as part...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: ...instrument used in this reconstruction lay the foundation for all subsequent versions of LSFM microscopes...

  11. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified...method allows for the recovery of a vast amount of data from a single experimental setup. As we’ve seen ...

  12. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ... in medicine, 27% in natural sciences, 82% in humanities, and 32% in the social sciences go uncited. Creative...the Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications ...example, learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about...at a time – A video by LeeAnne Thayer, a Ph.D. candidate in marine sciences at the University of Maine,...

  13. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for...Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik January ...

  14. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...genetic screening. gRNAs targeting every gene in the human genome for example, can be made easily and cheaply...Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle...through the creation of innovative technologies. To date his group has develop several popular CRISPR-based...

  15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...expression atlas of miRNAs and their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878....methods are often quicker and the repositories of validated small RNAs are vast and difficult to beat in terms...knockdown cells lines, and you can usually find validated shRNA sequences for your intended target. RNAi...(2021). Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Molecular Cell...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... al. "Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7... in fusion proteins. Since GFP is such a well-validated, all-round good performing probe, it is the first...components. For a review see Lotze et al. (2016). To date, small probes that are entirely genetically encoded...

  17. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison of different...how they can be used, and how they’ve been used to date . If you have any questions, please write to us ...time, Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This strategy allows...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...W, Khalili K. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci...approaches, and each of these gRNAs will need to be validated for high on-target binding and low off-target ...Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research...

  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...antibodies against all human extracellular proteins with community-based validation. They also have plans...additional validated reagents, and, with these reagents in hand you’ve got a good foundation to begin experimenting...researchers to share data thereby enabling you to do modified analyses on published data if you need to. Similarly...share data through their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all...biological researchers deal with larger and larger datasets and use computer based analysis techniques, an...allow researchers to share code they’ve used for data analysis while other organizations like Dryad make... of causation from initial hypothesis through validation are easier to understand. The need for great ...

  20. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...applicable to industry if this path interests you  (e.g. human disease or models of disease, drug properties, etc.... Gather data on the lab choices How do you find out what the lab is really like? Gather data of course...me when the most analytic scientists seem to toss data-driven reasoning out the window when making decisions...for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni as you can or schedule...meetings can be the most informative way of gathering data. Former lab members can give open, revealing information...humiliation, accusations regarding lack of effort, intimidating use of discipline or competence procedures Threats..., persistent teasing, name calling, insults, intimidation, sexual harassment Isolation – including preventing...
Showing: 221 - 240 of 256 results