... expands learning outcomes by emphasizing the importance of critical thinking, persistence, teamwork, ...emerging scientific field, BioBuilder embraces new opportunities for scientific exploration, discovery, and collaboration...with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group meetings and...the BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist ...on the Addgene Blog Synthetic Biology and the Importance of Assembly Standards Synthetic Photobiology:...

...it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent... lobes laying open like a book (Figure 2). The portion of the CTD that recognizes the PAM is so flexible..., but they improve cutting efficiency and are important for robust activity in cells (Jiang & Doudna, ... guide RNA stabilizing the DNA-binding protein portion of the CTD, ready to check for PAM sequencines....correct positioning of the non-target DNA strand is important for stabilizing the active conformation of the... University of California, San Francisco, with support from National Institutes of Health R01-GM129325...

... non-linear dependence on excitation power is important since different fluorescence imaging strategies...requirement for quantitative live-cell imaging, it is important to be able to quantify the photostability of fluorescent... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence.... To avoid changes in excitation power, it is important to perform the experiments on a single day on ...

... since I was still in grad school, I had the opportunity to take a course through the school of journalism...donors such as fundraising materials and progress reports, in addition to articles for the Fred Hutch website...tough assignments, the skills that Susan  finds important for her work include: “Listening. Evaluating evidence...started in science communication: “Find as many opportunities as you can to get your name on things.” Susan...this easier to do while still in school since opportunities, such as writing for a student-led publication...

... how these structures are generated, why it’s important for cells to sense their formation, and how clinicians...in turn lead to genome instability. Thus, it’s important to strike a balance between productive levels ...be delivered or anticipating this response are important considerations when using these reagents. RNA...recognized by RNase H which hydrolyzes the RNA portion, allowing the DNA strands to re-pair. The two dominating...stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease...

...As a scientist, perhaps the most important part of your research is communicating the results of the ...the work. Yes, those control experiments are important - but without letting other scientists know the results...Some organizations, including some journals, also support open peer review or curation models where editors...earlier attention and feedback giving authors the opportunity to strengthen the manuscript prior to submission...Along with other open-science organizations, we support scientists in sharing their results and materials...

...different ways depending on the team. When Tony, our corporate counsel, used the word “service,” did he mean ...mean it the same way as Bryan, our customer support manager? When Gizela, the aforementioned lab manager,...terminology of a new field. Annotate: When creating important documentation, including SOPs or project-related...Product Manager role at Addgene is “Words are important!”  If you try any of these approaches in your ...propensity for cross team communication, that’s an important soft skill to carry into the next phase of your...

...any other important features that differentiate this plasmid from its predecessor. Important features ...restriction sites used during cloning, and most importantly, the predicted full plasmid sequence. Other information...sequence verify the plasmids. This is the most important step in ensuring quality, as it will catch any...sometimes have to design new primers to sequence the important features. You can use primer design software such...

...experiments are important for visualizing ncRNA localization and function, but directly incorporating very long...transcribed but do not encode proteins. They play important roles in many cellular and regulatory processes...complex and not yet fully understood, so it is important to carefully select the target locations of knock-in...nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery of a target...

...gene incorporated into their genome. While transgenic mice have the new gene construct incorporated randomly...embryonic stem cells. Non-homologous recombination incorporates this DNA randomly into the mouse genome. In ...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...vectors used to create knockout mice include a reporter gene, which replaces the targeted gene and provides...

...around the world, the spike protein has had many opportunities to evolve. Several variants are now circulating...different spike protein variants that can be incorporated into pseudoviruses. The lab used these pseudoviruses...Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung epithelial...). In their search, they found mucins have an important role in viral entry, which are inhibitors of SARS-CoV...research through sharing reagents has never been so important.     References and resources References ...

... sections as a primary research article, it’s important to understand that presenting your work in poster...certain posters resonate with you (or not). It is important to reflect on these impressions when thinking ...concise as possible, communicating only the most important results to your audience. With a poster, you have...particular styles recognized to work well. It is important that your readers can navigate your poster and...prominently, making it the star of your presentation. Portrait. This style displays a poster vertically rather...

...these tough issues is essential for building a rapport with these communities, which is in turn crucial...epigenetics. So, I sought out a number of different opportunities to broaden my training beyond the biological...policy-related topics, as time permits. Also seek out opportunities to communicate science to non-technical audiences...either giving or running these events will be important both in preparing you for future work in these...communicate your work to a non-technical audience is an important skill for every scientist. After all, scientific...

...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...degradation, and is integral to the nuclear export and translation processes as well.   Figure 2... non-poly(A) transcript-stabilizing and nuclear export elements such as WPRE (5) and CTE (6), and/or use...the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1 Gag expression ...

... I can support that.” That was interesting. If people could be against GMOs as food but support GMOs that... up for real: bank account, business cards, incorporation, laboratory lease, etc. I still flip to that...result in a new utility, was there a business opportunity out there for us? After about a month of discussion...color, but the experience is invaluable. This opportunity has given us the chance to develop the technology...

...plasmids/year from 2017 to 2023. This growth is supported by new labs joining the Addgene CRISPR community...year of deposit. Outlines show a representative proportion of CRISPR plasmids requested, categorized by ...plasmids deposited by country; square size is proportional to number of plasmids deposited by each country...plasmids requested by country; square size is proportional to the number of plasmids requested by each ...Barrangou for his authorial contributions to, and support for, this and previous Addgene CRISPR publications...

...builds on existing popular web services, currently Crispor and Crispresso, that adhere to the same principles...decided to adapt the popular guide design tool Crispor by Max Haeussler. With a few extra lines of code... to analyze  CRISPR repair outcomes, which is important for quality control. The only ones we found that...analysis because of its ease-of-use and comprehensive reports. With CrispyCrunch, you get the all information...coding region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN)...

...Characterization of the Pink and Blue Pigments of Pocilloporid and Acroporid Corals. The Biological Bulletin 189:288...chromoproteins and fluorescent proteins, it’s important to note that despite their distinction by name...interactions with cytosolic proteins. This step is important as non-specific binding to proteins can inhibit...improved expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604...

...detector using a series of mirrors and filters. Importantly, conventional flow cytometry uses one detector...broad, detectors will usually only pick up a small portion of that spectra to use for compensation.   ... flow cytometry for a given experiment, it is important to consider how many parameters you want to use...conventional and spectral flow cytometry, it is important to consider the expression levels of the markers...two highly overlapping cellular markers, it is important to pair them with two fluorophores that have distinct...

...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...), the same cloning procedure can be used to incorporate each element into the final construct. This strategy...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...sequences), so enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek...