... all the boxes’. Once you’ve assessed your skill set and done your research, it’s time to start sending... a project, overcoming inevitable failures and setbacks. There are many roles that actively require these...

...polymerase using your favorite PCR protocol.  2. Set Up TOPO Cloning Reaction: Mix together the PCR product...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

...must be evaluated on a case-by-case basis. While setting up their CPP system to study protein transduction...

...with a different dCas9 binding module, alternative sets of transcriptional programs can be layered with ...

...then the chance is that you have handled large data sets and are familiar with statistical analysis. If you... If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers usually don't...

... and detailed protocols on topics such as how to set up your digests and pouring/running a DNA gel.  ...glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a visual reference...

... to create a fully controllable and reproducible set up,” Bernach explains. Developing an artificial leaf...

...they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that contain two hairpin sequences..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...

...mutation rate by only ~2-fold over nCas9 levels. Two sets of modifications were also made to PolI3M to increase...

...and plasmids to researchers in resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid...

... base editing and prime editing. How do you then set up an MBE system that contains a double-win for both...

...template for PCR amplification with a degenerate primer set to amplify IgG variable light (VL) and variable heavy...

... Yingxiao Wang lab.  The crux of the system is a set of heat-inducible lentiviral CAR constructs, the ...Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

...for team meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 ...

...and the CARE guidelines for case reports are two sets of guidelines frequently used by editors. Providing...

...series will cover 2 of the 7 mentoring questions I set out to answer. First, I will describe some of the...

...Buy American, Hire American" Executive Order which sets broad policy intentions directing federal agencies...ensure that each candidate meets the requirements set forth by USCIS before engaging with clients. To learn...

...that is, an antibody shown to perform well in one set of conditions may not perform as well in other conditions...cell types of interest. By correlating your data sets, you can increase confidence that the labeling observed...available. Various proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g...

...mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...

...Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...