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We narrowed to 456 results for: val

Showing: 221 - 240 of 456 results

  1. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...replicates and controls (see Tips below), and by validating leads with other methods. More common are false...your binding. And most importantly: Independently validate identified binding partners with other techniques...

  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...simple and efficient way to overexpress a gene and evaluate its function. In nature Agrobacterium contains...PMID: 19183892. De Ruijter, N. C. A., et al. "Evaluation and comparison of the GUS, LUC and GFP reporter...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...two direct repeats which can be used for marker removal so that the marker can be reused to introduce multiple...recognized and cleaved by RNaseIII resulting in cell survival. Plasmid addiction systems can be great additions...

  4. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... BK (2019) Rett syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2...(2017) Improved MECP2 Gene Therapy Extends the Survival of MeCP2-Null Mice without Apparent Toxicity after...

  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... been full of bad news and dreadful prognoses. Survival rates for mesothelioma are creeping upwards, and...greater impact on the life expectancy and overall survival of mesothelioma patients. However, no matter how...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Maine, about her research on plummeting embryo survival rates. Video on We Share Science: http://wesharescience.com...audiences that may otherwise never learn from the valuable research we are doing. Browse the site, learn ...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...output to link the processes correctly. After this validation check, we repeated our strategy and copied a ...the Addgene blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...accessible. The yields from magnetic beads are equivalent, if not slightly higher, than those of agarose...References and resources References:  Björck L, Kronvall G (1984) Purification and some properties of streptococcal...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of the reasons ...https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...from Addgene!   Figure 1: Experimental validation for Anti-V5 [IPI-SV5-Pk1] in a western blot assay...efficiency of this dual-plasmid approach makes it a valuable resource for advancing our understanding of its...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...click chemistry reactions instead of biotin — a valuable development in and of itself. After adding one... reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D Atlas.  ...

  13. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...career advice, open doors for you, and teach you valuable lessons along the way. We think it’s so important...in lab to go to with questions and concerns is invaluable. With a little thought you can build meaningful...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas...0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid vectors for...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... line across the peak will indicate the cut-off value for a positive signal (Figure 8). Note that the ...Huwait, E., Gari, M., & Al-Qahtani, M. (2018). Evaluation of in vitro chondrocytic differentiation: A stem...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...whacky cell lines”. This may certainly have been a valid concern for the discipline (or lack thereof) in ...colleague?”. The main difference is the QC and validation carried out by the commercial repository. Repositories...

  17. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...notebook starts with defining your lab’s needs The values of ELNs show in the long run as they enable researchers...part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities such...

  19. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your gRNA Additional ...Plasmids for Plants Read Our CRISPR Guide Pages Find Validated gRNAs for Your Next Experiment ...
Showing: 221 - 240 of 456 results