We narrowed to 783 results for: INA

... EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional control...Flp-expressing AAV. See our recombinase AAV inventory here New recombinases Plasmid Serotype Name ... µL size serotype testing) pAAV-hSyn-EGFP Recombinases Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo...

...yeast strains S. cerevisiae and S. pombe have dominated the research scene. But what about the other yeasts...promoter, AOX1, was co-opted for the expression of recombinant proteins by the pharmaceutical and biotech industries...efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t ...secrete its own endogenous proteins, making the recombinant protein its major secretion. Beyond sheer protein... good for post-translational modification of recombinant protein products, specifically glycosylation....glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast ...phaffii, relative to S. cerevisiae, is that it predominantly uses non-homologous end joining (NHEJ) to perform...

... TadA-7.10 version with a faster deamination rate so that deamination can happen before Cas homologs let...fused to a DNA-modifying enzyme, in this case a deaminase. Base changes from C•G-to-T•A are mediated by ... modifying enzyme to the target site, and the deaminase induces the DNA base change without a DNA double-strand... is ABE7.10 which contains the deoxyadenosine deaminase, TadA-7.10 (Gaudelli et al., 2017). The idea was...stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the...much higher base editing activity compared to the original TadA-7.10 enzyme. After testing numerous TadA ...10.1038/nature24644 Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW,...

...anterograde tracer) or upstream from the axon terminals to the soma (retrograde tracer) (Fig. 2). Viruses...only become activated in the presence of Cre recombinase, which is limited to the hippocampus. Thus, only...titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause challenges ...transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia...impact of this phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce...projection pathway neurons and then inject a recombinase-dependent chemogenetic vector directly into the...that are physically blocked, such as those in the spinal cord. Now that you understand this new tool, go...

... signals from time to time. False positives in original studies and false negatives in replications are...powered research designs. Unanticipated discoveries Finally, there are good reasons for obtaining a different...replication study. An important difference between the original setting and the new one, previously unaccounted...different handling can all be important.  If we eliminate the problems above: unclear methods, QRPs, and...when replications do not come out the same as an original research finding we can be more certain that some... papers. The precise timing, order, or every combination of materials and treatments is all too often ...reports, the gold standard for testing hypotheses Finally, Registered Reports combine all the best practices...

...and may increase the risk of mistakes or contamination. Finally, choice of concentration method depends...RCL). Replication competence can arise from recombination events during viral production and poses a biosafety...elements across multiple plasmids, the risk of recombination and consequently, the generation of RCLs is ...plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems...additional plasmids thereby increasing the number of recombination events that are required to generate RCL. To...volumes accordingly. While you may need to make some final adjustments, this is generally a good place to start... Keller B, Makalou N, Sutton RE. Systematic determination of the packaging limit of lentiviral vectors...

...list of Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism...depicted. (Bottom) Schematic depicting available destination vectors for various delivery methods, along with... with compatible inserts. All destination vectors contain an ampicillin resistance cassette and an origin...components you want, simply assemble them into a destination vector via Golden Gate cloning, perform an exonuclease...sequence for every individual assembly. What destination vectors are available? What you’re building ...Organism Delivery Type Destination Vector Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based...type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options depending on above selections...

...and Shannon Rinaldi in the Addgene lab! Read their Instagram profiles here (Kenkel, Rinaldi)! But leaving...might not be the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why ... have to go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her...science, she stuck with it. Lab Manager Shannon Rinaldi values loving what she does. Her parents told her...response to this question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the...came to Addgene with a goal to learn nonprofit finance, and while I've done that, my big takeaway has ...conversation master knows that although her focus is finance, it is just as important to bring love and fun ...

...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017...plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish the...libraries are then QC’d, pooled, and prepared for Illumina sequencing on our newly donated MiSeq.  For Complete...electrophoretic gel, and qPCR quantification of the final libraries. A broader view of the whole plasmid To...plasmid.     Confirming tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral... also adds the necessary adapter sequences for Illumina sequencing as well as unique barcodes per sample...

...unique repair templates. A final ligation reaction correctly assembles the final targeting vector (Fig. 1...need for PCR or cloning, as digestion of the destination vector with SapI yields 2 sites- the first site... the same unique SapI 5’ overhang, different combinations of connector and tag donor plasmids from the... a tissue specific manner, as well as 3-site destination vectors for inserting a tag at multiple target...the Hornung Lab, allows users to easily create C-terminal tag fusions of endogenous genes in human cells...plasmid containing the homology arms and an N- or C-terminal tag. The two PCR reactions are then mixed and ...expressing Cas9. This PCR toolkit offers C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5...