We narrowed to 821 results for: LAS

...hot plasmids in our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast...podcast! Hot plasmids covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly...In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins...Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons...Listen to Our Segment on these new AAV Vectors Hot Plasmid 3: mCyRFP1 - a new, monomeric, and cyan-excitable... protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent...Receive Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...

...We've developed a new plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we...scale laboratory-based functional experiments that classify which variants are likely gain-of-function, loss-of-function...correlations between variants in a gene of poorly classified function and a well established cellular signaling...phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome this first challenge, we...developed the Broad Institute Target Accelerator Plasmid Collections, now widely available to the scientific...in our recent publications, we used the cancer plasmid collections listed above to demonstrate how gene... hope that by releasing these new and exciting plasmid collections (together with the data generated from...

...you encounter mycoplasma contamination. Causes of mycoplasma contamination The mycoplasmas enter the cell...removal of mycoplasma but, can get rid of most of mycoplasma contamination and are the last alternative... growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation...suppress the growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture...highly expensive). Detecting mycoplasma contamination Detection of mycoplasma by naked eyes or optical microscope...most of the mycoplasma species, apart from a few of them. Another method used for mycoplasma detection ...infecting mycoplasmas are used. A broad range of primers encompasses almost all the infecting mycoplasma. The...

...Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important...of purity. Based on these premises we can classify a plasmid preparation in 3 different ways: Transformation... into a plasmid backbone. Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian... an external plasmid in mammalian cells and it will likely require micrograms of plasmid DNA for each ...larger scale, to allow the extraction of enough plasmid to last for multiple experiments. Another fundamental...utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation ...Addgene blog Browse blog posts on plasmid cloning Read our Plasmids 101 blog posts Learn about the differences...

...fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during plasmid DNA ... a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org...Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...Does it make a difference? Short answer: yes. This last step will influence the stability of your sample...Nowadays, the most common E. coli strains used for plasmid amplification are endA negative, meaning the gene...degradation and which experiments you can use your plasmid.  DNA elution choices: TE, Tris buffer, or water...polymerases. In the situation where you need to use plasmid in TE for any enzymatic reaction, an effective ...

...viral vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...contain the full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12 kb...in my samples? Plasmid recombination can affect viral vectors (LTRs and ITRs), plasmids with multiple ...other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids and ... length plasmid. If you find that your bacterial cultures contain a mix of full length plasmid and the...smaller, recombined plasmid around 1.5 kb. When cut with BamHI, the expected linear plasmid is present, but...get to see everything that can go wrong with a plasmid. One of the most frequent problems that I help ...

...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...30, 2020 and last updated Jul 12, 2021. What started out as a small collection of plasmids for coronavirus...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...scientists through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the...

...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs...true biological insight. Pooled and individual plasmid formats A first for a library at Addgene, you can...first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes, ... screening in a pooled setting. The individual plasmids will also be useful for imaging or gene expression...assays necessitating the screening of individual plasmids separately. Additionally, this set can be used... Page Find Validated gRNAs Deposit Your CRISPR Plasmids ...

... from Addgene Plasmids 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing... on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid has been...your deposited plasmids Increased visibility of your plasmids in the "Associated Plasmids" lists Benefits...Find other related plasmids in the "Associated Plasmids" lists Properly citing plasmids you've requested...Citing This Plasmid" list. Email us at [email protected] with the Plasmid Name, Addgene Plasmid ID #, and...'ll find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in...explore the TetO-FUW-OKSM plasmid page. Or search for your favorite plasmids and see where they've been...

..."Addgene is one of the leaders in distributing plasmids amongst the academic community. On the BioBricks... with Endy more about the BIOFAB and BIL gates plasmid kits, the Biobricks Public Agreement, and the future...first to be made available via Addgene and the BPA Plasmid Collection - enable? Endy: The BIOFAB collection...engineering an entire cell. Typically when people use plasmids, they are trying to do one thing at a time. What... tricky to tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic...