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...Each round of amplification should result in a 10-100-fold increase in virus. Purification (2 days) - Purification... Purification is required if rAdV is to be used in vivo. The standard method for purification of rAdV ...or 10 days) Physical titer (particle count, rAdV genomes/mL) can be measured by: Optical density: particle...vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high...AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years...years since its discovery, researchers have done significant work to improve the Adv genome’s utility in research...Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new...

... capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold switch...portable quantification of diagnostic results.  Our approach now detects samples at clinically relevant...global strains of the Zika virus (Asian-, African- and American-lineages). Strain discrimination is especially...current outbreak because of the neurological symptoms associated with the American-lineage of the virus. For...populated regions of South, Central, and North America. Diagnostics will play an important role in helping...outbreak was reported, and we felt that, given the critical need for low-cost, field-based diagnostics, our... journal Cell just six weeks later. The Zika publication timeline I think we can speak for all the authors...

...methods of replication. In fact, an entire classification scheme, known as the Baltimore replication classes...that in a bit! Viral genomes and replication When it comes to replication, viruses tend to march to the ...developed around how they replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: ...an intermediary DNA replication step between DNA and mRNA. DNA genome replication can involve an RNA or...that have been genetically modified to either limit or completely eliminate their replicative ability. The...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...cell. The cell then uses its own machinery to replicate the viral genome and proteins. This is, for the...

...understanding of complex biological processes. Many tools exist to perturb biological function in a direct...use in dsRNA applications. Figure 1: Schematic of an RNAi-inducing transgene. Typically, the desired...individually engineered and empirically evaluated for their gene silencing efficacy, thereby requiring a substantial...limitations of any technique in the context of the biological question. For example, while gene knockouts have...makes traditional knockouts unattractive from a practical perspective. Therefore, techniques that function...degradation, blocking translation, or chromatin modifications of complementary gene sequences (Figure 1). ...silencing can be easily induced by exogenous application of long or short dsRNA. However, it is important...

.... The overlap here is that it is unethical to use scientifically unproven technologies to edit a human...human clinical trials. This model also demonstrates that when we are at the point of scientifically rigorous...throughout the history of medical research, there have always been people who unethically experiment on humans...Cas9 genome editing is not far enough along, scientifically, to be doing in humans; We have not had a broad... recently published a report on the social and ethical issues of genome editing and human reproduction...with healthy mitochondria, with the intent to eradicate mitochondrial diseases. The key success of the...investigate the scientific possibility, safety, and efficacy of the mtDNA transfer options being investigated...

...cell lines (European, East Asian, Indigenous American, Africa, or South Asian) used in the screens based...Sean and Aaron knew that their study had significant statistical limitations, and expected to find only ...kind of biological framework, suggested to them that their findings may be the result of a technical artifact...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...Dependency Map cancer cell lines (left). Heatmap indicating the breakdown of ancestry associated dependencies...Seeking an explanation  First, they looked for a biological explanation, a relationship between the genetic...machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA target sequences...

...next logical step after technical mastery. However, being a great scientist doesn’t automatically translate...cohesive team environment. Neglecting communication skillsClear communication is the backbone of effective management...transition While a PhD equips you with exceptional technical skills, transitioning into management often means...stepping into unfamiliar territory. Unlike solving technical problems in the lab, management involves a different...time, I discovered that I not only enjoyed the technical aspects of my work but also found fulfilment in...your team's—can lead to frustration. Clinging to technical tasksDelegation can feel unnatural for those accustomed...myself to focus on my core responsibilities, which typically revolved around the broader vision rather than...

...weather befitting of the ominous political climate in which American scientists find themselves today,...we spend years being taught to communicate in a very specific, technical manner, so much so that we often...also a science communicator, UBC Public Scholar, and a member of the Vancouver SciCATs. Follow her on ...March for Science from researchers all over North America. I hope that these perspectives, the optimism they...Alina Chan is a postdoctoral researcher at Harvard Medical School working on human artificial chromosomes....election.   Even as we marched in solidarity with our American neighbours, the fight is far from over here at...April because devaluing science has alarming implications for our public policies—many of which impact...

...the IPI Glypican Collection here! Figure 3: General glypican domain structure. Glypicans are proteoglycans...recombinant antibodies targeting Glypicans By Ashley Waldron Glypicans are important modulators of cell...AAV injection at 4 weeks. Cortical layers (L1-6) and scale bar are indicated. B) Specificity, as percentage...Here's what you'll find in this post: Glutamate indicators with increased sensitivity and tailored deactivation...lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR Dynamic protein labeling...viral preps targeting interneurons Glutamate indicators with increased sensitivity and tailored deactivation...and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, and sensitivity...

...your specific application. Consider also that there may be variations within an application. Take immunohistochemistry...website highlights a variation of your application, a publication successfully used it in a similar way...exciting new project aimed at understanding how a critical cellular pathway is regulated. You’ve read all...choosing an appropriate antibody first look at the application-specific data provided on the manufacturer’s ... a linear antigen tend to work very well for applications that denature a protein to its primary (linear...the most similar to yours. Take a look at the publications cited on the manufacturer’s website, which may...plan to. To secondary or not to secondary?  The application will also determine whether you should use an...