We narrowed to 402 results for: LARGE;
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TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...
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Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m.... advantage of this technique is the potentially large number of single clones obtained (on occasion we... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....and actual molecular size – it is possible that a large or charged FP fusion can change the subcellular ... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....relies on antibody chains, which are relatively large and are not expressed consistently throughout cells... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....Permeabilization What is the point? Antibodies are large proteins that need a little help crossing cell membranes... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....proteins are split between strand 10 and 11 to yield large (1–10) and small (11) fragments. In several cases... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....amplicons. Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m.... prone to recombination, while a library with a large backbone might be more difficult to transform. ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m.... This specific example is attractive due to the large number of sheer molecules and signal you can generate... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m..... Picking receptors The receptor you choose is largely dependent on what cellular response you aim to ... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...the insert site. A positive clone will produce a larger size product then a negative clone without the ...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m.... parts. That was the motivation for releasing a large parts kit. Once you have so many parts available... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m.... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced ~1,500... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....insertions and deletions (indels) most likely to have large phenotypic effects such as those that affected gene...