We narrowed to 641 results for: Ncl

...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

...control Historically, AAV vector QC has been sparse including only titration of AAV particles and a protein ... the expression cassette that we want, it also includes a small amount of spurious DNA from the packaging...

...but suffer from several important shortcomings including low processivity (ie. the probability of the polymerase...transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP ...

...module. As a general guideline, experiments that include growing plants require pots, trays, soil, fertilizer...efforts. Our education partnerships have grown to include local school districts, professional education ...

...standardized methods for evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise...guest bloggers Dominik Paquet and Dylan Kwart, including a detailed look at how cut-to-mutation distance...

...postdoc Frank Graham’s 293rd experiment, hence the nomenclature we use today: HEK-293. The exact origin of the... option! Especially if your research interests include receptor biology, ion channels, or involve human...

...are some highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, ...sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and ...

...and is based on the pTRC99a plasmid. It does not include the PBAD promoter. If you already have a pBAD33...the plasmids deposited from Di Ventura's lab, including the popular light-inducible nuclear export system...

...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately... plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition...

...month.  We are currently focused on two projects, including one led by a talented biomedical engineering team...still able to get through to a number of experts, including Harvard/MIT faculty, venture capital and angel...

...the specificity of their luciferase assay, they concluded that components in the buffer used for protein...works in multiple types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, ...

...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit...RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can...

...creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel valued...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based...

...navel-gaze over from the last couple of weeks, including whether or not scientists can recognize so-called...work on emerging issues in novel technologies, including self-driving vehicles, synthetic biology, biosecurity...

...of physical materials that we use in our labs, including plasmids. All plasmids at Addgene have unique ...materials and methods section have been updated to include the option of citing with resolvable compact identifier...

...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated...

...researchers explore many aspects of ApoB-LP biology, including quantification of abundance, size, and localization...made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors...

... browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for...best serotype for their particular study, which includes comparing the transduction patterns of different...

...pivotal role in an array of biological processes including development, the immune system, and cell turnover...been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that ...