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We narrowed to 807 results for: APP

Showing: 361 - 390 of 807 results

  1. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...a plasmid or viral origin. The type of cloning approach you choose ultimately will influence the quantity... for the extraction. Restriction enzyme based approaches can require several hundreds of nanograms or ...assembly or Golden gate assembly use a more direct approach, allowing scientists to clone from a smaller amount...

  2. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...Citing This Plasmid" database - and we always appreciate any help we can get. If you know of an article...PMID) for the article that has used this plasmid. Happy researching! If you need any assistance finding ...have made to the repository, email us and we'd be happy to help. Read More Tips from Addgene Plasmids ...

  3. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...looked at the trace you would be happy. But look closer, the overlapping peaks in the chromatogram suggest... or consider asking your sequencing facility to apply a special protocol for a difficult template. Anything...

  4. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic...feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking for CRISPR...

  5. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...don’t think you’re qualified. The worst that can happen is they say no, which is infinitely better than...good, customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there...

  6. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... The new tool was developed and implemented by applying the Golden Gate cloning method to human proteins...says the new tool might even find therapeutic application in addition to their use in basic biology, for... make the library even better, we would be very happy to share updated versions of the kit.” Reference...

  7. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to prepare....org.  Thank you again for a fantastic year and happy reading. Best wishes on your research in 2020! ...

  8. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the 70,000 or more...community will find these reagents to be useful. Happy screening! Many thanks to our guest blogger, John...

  9. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...and fusions represented in this collection, approximately 80% (over 800) of them had not yet been functionally... viability compared to a wild-type control. Approximately ~22% of the mutations, however, significantly...

  10. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...ve also carved pumpkins (yes, Blugene made an appearance there too) and shared fascinatingly creepy science...defective exoskeleton formations. These genes were appropriately named as spook, spookier, phantom, disembodied... grasshopper harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B: Biological...

  11. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh...

  12. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... size and frequency [3]. There are several applications in which the production of deletions by paired... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

  13. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...were willing and able to listen to Addgene’s new approach of working with the industry-based scientific ...reduced to days and weeks. As of June 2020, we are happy to have the support of the following organizations...project is complete, Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research...

  14. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ... value and priority put on good mentoring and applauded the new requirement for PIs to include in their... of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...opportunities.  Scientists must do science to be happy so the beginnings of solutions can’t come too soon...

  15. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...expression level. Labs have been trying different approaches to improve CTC capture, but I hope that our antibody...

  16. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ... al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types...population, it’s easier to visualize individual/non-overlapping cells. While transgenic animals are commonly ...

  17. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...go-to method for mapping where a protein binds genome-wide, and has been widely applied in many model organisms... the chance of false-positives, as proteins may appear to be binding at particular genomic sites when ...

  19. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...called protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing... are recognized by the Cas9 protein from the appropriate species. Some Cas proteins, like LbCas12a or ...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  20. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...inflammation, and neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda...GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2 and Gβ constructs. Cells are then treated...plasmids in a number of different experimental approaches. They first assessed the activation of different...

  21. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...been requested 100+ times Since we so sincerely appreciate our depositor’s dedication to scientific sharing...plasmids with us. One way we can gauge if people are happy depositors is if they deposit plasmids more than...off! To share your photo, tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a ...

  22. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...as #happydepositors who stopped by to say hello. Needless to say we all got very good at applying temporary...six continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware...

  23. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... though not all, of these defenses. For some applications of genome engineering - such as certain forms...and improved SB transposase, SB100X, that is approximately 100-fold more efficient than the first-generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

  24. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...team. It is pretty clear that if people are mostly happy at work, they will work harder and contribute more...contribute to job satisfaction: A feeling of being appreciated and valued This often stems from being included... Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental...

  25. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... supplies every 10 years or so. Actually, I was happy to hear that I am not the only Addgenie who has ...I started looking for smaller and smaller gel apparatus until I settled on using glass slides. With just...this one only cost a few dollars.  I used this apparatus for 15 years before I hung up my pipets.   ...

  26. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...chatting makes it pretty easy to make 30 minute appointments with people all over the world. Think about ...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get...

  27. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...form has 5 general question types and it takes approximately 15 minutes or less if you have all of your data...under copyright, you will need to obtain written approval from the copyright owner and will have the opportunity...viewing the data you have any feedback at all, we’d appreciate you sharing it through this form. In honor of...

  28. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  29. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...Science magazine. That one I just could not resist applying for. Not that I knew anything about being an editor...constellation of interests and skills than what fits for applied research. I focus in general terms on a couple ...Review and Scientific Publication, where diverse approaches to peer review were discussed. Science communication...

  30. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...lack of site selectivity typically diminishes the apparent affinity for the ligand, especially if the modified...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which...
Showing: 361 - 390 of 807 results