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Addgene
Showing: 361 - 390 of 701 results

  2. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...interacting with people and talking about science makes her the perfect Addgenie and she can’t wait to see...even visit your lab! We look forward to helping you make some serious discoveries.   10 Years of Plasmid...

  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... a little thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity ...that is unlikely to perturb protein function. This makes it amenable to being left on your protein of interest...

  7. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ...

  8. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...in doer mode even late in our careers.  Managers make sure the work gets done Managers do this by removing...communication. A manager must both hire and fire to make sure the team is the best it can be. Managers must...

  10. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ... to share your science 1)   If you don’t already make it a habit to talk – really talk – about the research...science, they have good advice for anyone looking to make their science matter. If you aren’t ready for a ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...and numerous experimental design decisions to make, it makes sense that scientists, many of whom are venturing...

  12. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is... strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain are compatible...

  13. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...serve as a good mentor. To read more about what makes a good mentor, see the previous post in this series...posts in our Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring...

  14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with...

  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to go...

  16. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...science communication research.   This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first...

  17. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...was awed by the teamwork and initiative it took to make all this happen with 80% of the team working remotely...Protection Program loan and received enough funds to make most of our payroll for the spring and early summer...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...extra extension time after the last cycle of PCR to make sure that the "A" gets added to all PCR products... the standard ligation reaction is fairly quick, make sure you stay organized and prepare everything you...

  20. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...stop for refreshment along the way? What ecosystem makes your heart sing with delight? From developmental...other scientists, or science journalists, or policy makers. The term editor covers a wide variety of careers...

  21. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  22. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward...that with this award from Addgene, we’ll be able to make big progress in elucidating association between ...

  23. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

  24. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

  26. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

  27. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

  28. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

  29. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

  30. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...
Showing: 361 - 390 of 701 results