We narrowed to 435 results for: Trac
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TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....These wire models were useful in that they not only traced the 3-D path of the protein’s backbone in three-dimensional...
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Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....recombination can potentially cause you to lose track of your mutation. To maintain stable lines of flies... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....anemone’s red colored tentacles resemble worms which attract fish for them to prey upon (Figure 1). Figure... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....endogenous promoter, you can use expression GFP to track cells participating in development or other physiopathological... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m.... is sedimentation equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....an excellent resource that has gained a lot of traction with researchers. It is an excellent way to meet... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....generation of such resources in less genetically tractable organisms lags behind. BiFC can also be used to... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....dire than Zimmer could have imagined. Does this detract from the quality of the book? It doesn’t need to... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....expression for the data likelihood contains an intractable integral of the product of a Poisson and multivariate... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....providing DOIs, this information can be cited, tracked, and appropriately credited, broadening the knowledge... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m.... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....but they can also be expressed in bacteria and extracted with common protein purification methods. Read... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m.... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....place. When the PCR is complete, a droplet reader extracts the droplets from the plate and measures the fluorescence... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag. Table... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....functions in the hope of developing drugs against intractable diseases. Currently, a number of labs are using... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic gRNA) are derived from... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....properly. For example Pechmann and Frydman found that tracts of non-optimal codons are associated with specific... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....being clear about what was expected, and keeping track of speakers. During your graduate or postdoctoral... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....data for our DNA-based reagents. Not only do we extract as much information as possible from depositing... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....strengths. . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....in vivo transduction of AAV serotypes generally tracks between strains of mice. This suggests that a small... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m.... by your presentation, then you’re on the right track. Furthermore, practice getting back to your story... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....Confirmation Pick 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....the terminating nucleotide and records it in a “trace” file to determine the sequence. Figure 1...