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We narrowed to 435 results for: Trac

Showing: 361 - 390 of 435 results
  1. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...These wire models were useful in that they not only traced the 3-D path of the protein’s backbone in three-dimensional...
  2. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...endogenous promoter, you can use expression GFP to track cells participating in development or other physiopathological...
  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...
  4. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your...
  5. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events...
  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et...
  7. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...but they can also be expressed in bacteria and extracted with common protein purification methods. Read...
  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...place.  When the PCR is complete, a droplet reader extracts the droplets from the plate and measures the fluorescence...
  9. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...
  10. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...
  11. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...properly. For example Pechmann and Frydman found that tracts of non-optimal codons are associated with specific...
  12. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...
  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of...
  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...the terminating nucleotide and records it in a “trace” file to determine the sequence. Figure 1...
Showing: 361 - 390 of 435 results