We narrowed to 801 results for: DES

..., it’s essential to understand them in order to design the most specific assays possible. In this blog...-target effects should impact your experimental design differently. Yes, it is always better to reduce...introducing a specified edit). However, if your design does have wiggle room, you can minimize off targets...

...facilitate the selection of cells containing your desired edit. The selection is marker is then seamlessly...sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,”...Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: wanted and unwanted target locus modifications...

...experiments to minimize it. Overview of DIO/FLEx vector design Recombinase-dependent vectors utilize pairs of ...between them. Early recombinase-dependent vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF)...you intend to use performs in your system. This includes a thorough testing of both the serotype and promoter...

...good starting point for most researchers as it provides a list of putative off-target sites that they ... approach misses larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded...contains both the rare off target event and the desired edit is low. Therefore, unbiased sequencing may...

...tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger Lab designed 2 variants of EosFP that...to green). For example, mEosFP (named after the goddess of dawn in Greek mythology) switches from green...

...in the development of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary... in different cell types, available at www.crisprbehive.design. CGBEs fused to pioneer factors to improve...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ...

...second edition includes new and updated content in a clean and easy-to-navigate design. You can find it...

...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...on how Addgene has used Nextflow to improve and redesign our pipeline. In it, they break down the unique...

...#12DaysOfCRIPSR hashtag to share new CRISPR content designed to help you with your CRISPR experiments and to...CRISPR, Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of...

...distribution. Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents in your...materials quickly and easily. Learn more about using barcodes. Check out our “Lab Tip” filter on the blog...

...anniversary! We've done so much in the past two (2!) decades that it's hard to summarize, but we wanted to mark...experience we're sharing through blogs, eBooks, videos, guides, protocols, and of course, the friendly folks at...

...and find our guides, plasmid kits and other resources for microbial research. Kits, guides, plasmids, ...

...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...

...CRISPR 101 eBook. This new edition of the eBook includes the details on advances in CRISPR including: ...bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to ...

...10,000 plasmid deposits per year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach...globally. *All data presented in this blog post includes data until November 9, 2020. Between then and ...

...of lab materials to create their decorations! Despite all the craziness of 2020, for our Zoom #HolidayParty...microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com...

...below the ordering table. Click on it, select your desired serotype and volume, and submit your request.  ...simply request your plasmid of interest in your desired serotype, place your order when ready, and we’ll...

...and point out one or more additional posts that deserve a peek in case you missed them. July 2016 was a...Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET flurophores for a variety...

... the United States while caring for a patient, despite wearing protective gear, and now comes news of ...Virginia School of Medicine have also recently described how the glycoprotein transforms itself to escape...

...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...IT10 expressed well in cells and did not form undesirable aggregates. Using budding yeast as a model system...

...is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...

...knew very early on that I wanted to be an artist/designer. But there were a few teachers and 'guidance counselors...Don't worry about the rest.”- Jason Snair, Product Designer On the job search “Always go for the job you want...

... cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half...Harrington, Lucas B., et al. "Programmed DNA destruction by miniature CRISPR-Cas14 enzymes." Science 362.6416...

...characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many...

...light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn them on or off...News & Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First,...

...on the Addgene blog and have created and updated guides on various topics in biology over the past year...containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview...

...collect in those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental...Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

...choice picks this month, I’m picking two posts that describe methods to make single base edits in DNA. The ...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...

...LaFleur, et al. 2024.   The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer...blog CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing CRISPR Cheat...

...infection, she shared that tweet with Jan Zheng, a UX designer that she knew. “We had actually met at a lindy...to possess the correct phages will test. These includes labs with a large number of different phages or...

...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...

...interactive game that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you...all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the changes at...

...are the three main takeaways from the paper for designing experiments with AAV: 1. Getting your viral vector...AAV from our viral service can share experimental design and data. With these different approaches to open...

...new Help Center! Created using the same reliable Zendesk system that powers our email support, this portal...your name and email address, after which you can describe your issue and press send. Doing so will bring...

...Improving Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is...

...when a high degree of signal to noise is desired. It also provides an excellent additional measure of DNA...

...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then arg1 vesicles...

... favors the breakage of the hydrogen bonds, destabilizing the molecule and favoring the separation of ...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...

...optimized for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that...

...camelids too, though not just for the reasons the UN describes. In honor of the Camelid family, I wanted to take...of times on this blog over the years and have described how they compare to other antibodies and affinity...