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  1. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...recommend a few: -The recently established Ebola Genome Portal at the University of California, Santa Cruz, offers...

  2. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

  4. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering...

  5. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies really made...experience. I am thankful to have been given the opportunity by Addgene. I have met some of the most genuine...

  7. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...#ActualLivingScientists). We also discuss the importance of actionability on the Addgene blog and how ...The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer feedback...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ... please! At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween...body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...

  10. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could...wider audience for more than just its biological importance. More of Daniel's stunning work can be found ...

  11. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ... lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes. “When PhD...required to achieve success in the lab, it is important to take the occasional breather and acknowledge...

  12. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...physical scientists are underrepresented. It’s important to note that these results could be biased for...domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized ...

  13. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been difficult... are blocked by alpha1-AR antagonist. This is important because alpha2-AR can provide inhibitory feedback...

  15. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate... what a site offers to their users, and most importantly, allow those users the ability to quickly access...

  19. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...Addgene's Europe office no longer handles customer support inquiries. The office is now focused on scientific... first to be located in London and focused on supporting European scientists. Born a french frog, I moved...

  20. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough...

  21. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

  22. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...

  23. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

  28. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

  29. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

  30. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...: a method to genetically manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101...

  31. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

  32. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... models, toolkits like this one provide new opportunities for investigating genetic mechanisms related...

  33. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

  36. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...

  37. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

  38. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...Growth Conditions To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers...
Showing: 481 - 520 of 780 results