We narrowed to 803 results for: POR

...the phage recombineering machinery are first electroporated with pCas9. Then, pCRISPR is introduced along... a mammalian cell line (Qi et al., 2013). Two important features of this early system are its regulatable...Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014) Exploiting...

.... They also mutagenized the cytidine deaminase portion of the base editor to create SpCas9 base editors...off-target activity (Richter et al., 2020). This is important because ABEs have generally been slow, meaning...editing for plants Read about four base editor reporters Additional Resources on Addgene.org Catch up...

...reflections on the program are here. This was a great opportunity for Addgene to present our own data on plasmid...in advancing this field and how we are able to support rapid progress in all areas of research. We welcome...

...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...The Frequency Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that...

...India, and Brazil.  We've also created a robust support system for scientists using materials in our repository...repository, from our customer and science support to our efficient and easy tech transfer process to our...

...biology and how it speeds science. Chapter 8 - The importance of communicating research findings through social... singapore...

...Whenever possible, we love to give scientists the opportunity to share their knowledge. One of our goals is...social media outlets meaning you’ll have the opportunity to interact with and discuss your work with many...

...to meet the diverse needs of our community and support a wide range of experimental designs. Here are ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

...synapses distant to the site of injection with high temporal precision.   Table 1: New ready-to-use viral ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

... well as plasmids from Addgene to bring new opportunities to Ghanaian scientists Click here to listen... Kwabena's goal to build biological science opportunities in Ghana. 46:35 - end : Kwabena's time in Boston...

...repositories, such as Addgene and the DSHB, play an important role in scientific exchange. In addition to ensuring...provide standardized quality-control and technical support to the community. Thanks to Our Guest Blogger! ...

...identify what your plasmid contains and other important information. Tip: A lowercase "p" is often used...simple piece of information can often convey many important details. Once you know the backbone a plasmid ...

... list some easy but effective habits you can incorporate to reduce your lab consumption and increase your...in your daily habits can feel ineffective or unimportant. With so many big contributors to the sustainability...

...out our blog post on CRISPR software to see the importance of programming for scientists!). Careers that...scientist or a professor. There are amazing opportunities that are offered with a biology major.” After...

...~10 years! We're extremely proud to be able to support scientists by bringing you all the plasmids you...storing them, providing quality control and customer support, making plasmids easy for you to find and request...

... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...recommend a few: -The recently established Ebola Genome Portal at the University of California, Santa Cruz, offers...

...water of HR work. What do you think is the most important resource that Addgene provides the scientific ... how to operate during the pandemic, but it’s important to develop a plan for moving forward. Sooner or...

...containing these base editors Spatially and temporally regulated AAV-based genome editing In 2016, Jonathan...AAV-based genome editing system is regulated not only temporally with doxycycline, but it can also be regulated...

... genetic circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering...

...Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies really made...experience. I am thankful to have been given the opportunity by Addgene. I have met some of the most genuine...

... of who is paying for them. More importantly, there’s the opportunity cost of your time; an investment...

... please! At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween...body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...

...patient data to identify mutations of unknown importance in cancer Previously available methods for investigating...passenger mutations from the “active” drivers is important for the development of targeted therapeutic interventions...

...#ActualLivingScientists). We also discuss the importance of actionability on the Addgene blog and how ...The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer feedback...

...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could...wider audience for more than just its biological importance. More of Daniel's stunning work can be found ...

... lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes. “When PhD...required to achieve success in the lab, it is important to take the occasional breather and acknowledge...

...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...

...through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate... what a site offers to their users, and most importantly, allow those users the ability to quickly access...

...transgene cargo capacity, enhancing anterograde transport efficiency, and engineering viral capsids that...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...

...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been difficult... are blocked by alpha1-AR antagonist. This is important because alpha2-AR can provide inhibitory feedback...

...physical scientists are underrepresented. It’s important to note that these results could be biased for...domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized ...

... interests. We do this by promoting citation, supporting sophisticated search, and curating plasmid collections...other resources. If you think we’re missing an important collection or if you think a particular plasmid...

...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

...Addgene's Europe office no longer handles customer support inquiries. The office is now focused on scientific... first to be located in London and focused on supporting European scientists. Born a french frog, I moved...

... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough...

...capsid, “pumps” the genome into the capsid through a pore in the capsid. Rep proteins are also involved in...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

...   Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary...

...was developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (...: a method to genetically manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101...

...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

...screen results. Screenshots updated 2024. Importantly, metadata for each screen is captured by BioGRID...also captured as metadata.   Screen results are reported as the original quantitative gene-level scores...