We narrowed to 814 results for: Ran;

...each sample remain highly normalized across a wide range of input DNA concentrations. This process is explained...plasmids on a plate) will typically have a wide range of input quantities. This variation is problematic...produced from large numbers of samples with a wide range of input concentrations (via proprietary chemistry...differences in input concentration across a 100-fold range (1ng – 100ng) are normalized to two-fold variation...

...can feel lost. Use this post to explore the wide range of careers available to scientists and open your...carry out in-depth research is applicable to a wide range of industries and is a highly desirable quality ...SAS, HTML, Matlab etc) are highly desirable in a range of roles, including: Science Reporter Technical... Statistician Bioinformatician Science Writer Grant Writer Journal Editor Science Marketer Software...

...This method involves measuring the absorbance/transmission of light through a liquid to determine the concentration...spectrophotometer measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of...PicoGreen or SYBRGreen, are specific for double strand DNA (as compared to spectrophotometric methods ...However, the measurements are made in the visible range and can be read with a standard ELISA reader if ...

...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics. https://doi.org/10.7150/thno.43360 7. Yuxuan...

...ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell...PubMed PMID: 31026591. Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...emerging IVF techniques designed to prevent the transmission of mitochondrial disease;” The Nuffield Council...upon a moratorium until another meeting, three branches – a private funder, government regulators, and...question we are left with is not “what is the translational pathway for CRISPR/Cas9 research” but whether...

...lab uses cellular extracts to perform transcription and translation in vitro, and the new BioBuilder module...cutting-edge research projects in synthetic biology and transforms them into modules that students and teachers ...teams’ biological engineering designs and ideas ranged from a biological breathalyzer to pesticide breakdown...

...supplied with a luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal...luciferase has a low quantum yield, and when transiently expressed in cells, generates much less signal... principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...

...edit. Since exploitation of NHEJ to generate translational reading frame-altering indels for “functional... an unedited site distal tothe predicted double strand break, and a NHEJ/ “drop-off” probe designed to...to bind directly at the predicted double strand break site (Figure 2A). The single molecule resolution...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates...

...next interview or whether your receive the next grant you apply for. After all, the only value your work...high level to their team.  When you're writing a grant, this means convincing a committee that your research...collaborating with colleagues in other fields or writing grant proposals to be reviewed by interdisciplinary panels...communities gather at ComSciCon's local and specialized franchise workshops throughout the year.  Find workshops...

... sampling and inference-making. Even perfect transparent and rigorous studies will give false signals ...for Open Science in order to reward increased transparency and reduced bias in scientific research. These...include policies for publishers and funders in the Transparency and Openness Promotion Guidelines; preregistration...Registered Reports; and recognizing increased transparency with badges. Before coming to the Center for...

...factors can negatively impact both translation and fidelity of translation, leading to system-wide effects... incorporation of an unnatural amino acid in translation (Noren et al., 1989). In the late nineties, this...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into ...

...DNA. The other component of these systems, methyltransferases, methylate DNA at particular sequences to...restriction modification systems containing methyltransferases that add methyl groups to specific DNA sequences...overlaps with the XbaI cut site (depicted as an orange line) because the restriction site is followed ...plasmids with no methylation (there are no methyltransferases in the PCR reaction). The template plasmid...

... and the nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method...containing the desired mutations, to amplify both strands of a plasmid template. In addition to containing...template plasmid, these PCR products are directly transformed into bacteria expressing recombineering proteins...fine, especially using plasmids in the 10-12 kb range. REPLACR-mutagenesis also requires making your own...

...how they started their company here. The first transgenic plant was engineered over 30 years ago, but plant... still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...s made even more difficult by the fact that transformation methods have also been patented. In order to.... We will also be working with open source, transformation technologies like those pioneered by Ewen Mullins...

...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...emission spectra. Component Excitation Emission pH Range DsRed.T3 488, 543*, 568 nm 587 nm ~4.9 - ...mechanisms behind autophagy by tracking what’s being transported to the yeast vacuole (i.e. cytosol, mitochondria...

...expense, and less of being left with a bunch of random solutions when all the spin columns run out. In... inherently not as stable as DNA- it is single stranded and its ribose groups are susceptible to hydrolysis...effectively isolate intact RNA, a fast, strong protein denaturant would be required- something that broke down ...Chirgwin and colleagues showed that a strong protein denaturant, guanidinium thiocyanate, did just this (Chirgwin...

...and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing resin. The protein...polyhistidine containing contaminating protein (orange). A low imidazole wash buffer disrupts the weak...polyhistidine-metal ion interaction is not disrupted by denaturants (Hochuli, 1988). In some cases, proteins can ...tag and then refolded by gradual removal of the denaturant. Not all proteins will refold once denatured,...

...technical support Our customer and technical service brands continue to be impeccable. We score ~99.9% helpful...lauded by another set of stakeholders - the tech transfer community. In a recent law review article we received... have a UI/UX team. Last time I checked we were ranked as the 21,806th most popular website in the world...pages and excellent work on our external website appearance and functionality are happening and this strengthens...

...the specific reaction you are running. Transformation Transform a few microliters of your ligation reaction...The PCR could fail to produce a product, the transformation may not result in any cells, or all the colonies...fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial strains...

...conference poster or talk, or submitting patent or grant applications; to informal settings like lab meetings..., and increase their impact. Open access and transparency Another major benefit of preprints is their ... of listing “Submitted” articles in their CV or grant applications. A number of organizations (such as...be prone to biases or errors, with a lack of transparency in most review processes. Preprints and their...

...expression in E. coli. This operon is under the transcriptional control of a strong constitutive promoter (... required enzymes, including a glutathione S-transferase homolog from a Janthinobacterium sp. Moreover... protein kinase A (PKA) activity at the plasma membrane in living cells using a construct in which TagRFP-T...used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime imaging microscopy...

...Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system...AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression...   Drosophilia Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options...

...chain and light chain for the antibody and when translated into protein will assemble into a fully functional...synthesize the genes that encode those amino acids (Tran et al., 2016). Each antibody would then be tested...P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies...:633–644 . https://doi.org/10.1083/jcb.201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) ...

...fall within a 10 fold range. Plasmids that don't meet the lower bound of this range are “failed” and removed...mutations, we check to see if they affect the translated amino acid. We also confirm that the species ...2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863...

...production of dsRed E5. This timer predictably transitions from green to red fluorescence (500 to 580 nm... of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP timer ...fusing Medium-FT to LAMP-2A, a lysosome-associated membrane protein, and following its subcellular localization...whether or not newly synthesized or long-lived transcription factors control gross changes in gene expression...

...would at least be guaranteed a successful result? While there are never any guarantees, some cloning techniques...Resources” section at the end of the post! Speed: Your grant deadline is approaching or your advisor is demanding...your insert, DpnI digest away the templates, and transform the PCR products into E. coli. Ligases within ...

...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...Cultured rat hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation ...via the Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether, if...kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option. Find ASAP5...

...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...and proteins that forms chromosomes. These long range DNA interactions are often detected with chromosome...control gRNA or predicted gRNA off-target sites. Long-range chromatin interactions identified with the multiplexed...

...during ESC isolation. Low Chances of post-transplantation rejection when used therapeutically High Low...similar functional utility, iPSCs bypass the transplantation compatibility issues and ethical concerns of... used therapeutically, the chances of post-transplantation rejection are low. Further, as iPSC generation...somatic cell type to another, also known as transdifferentiation.   Many thanks to our guest blogger, Kusumika...

...blastn), Standard Protein BLAST (blastp), and Translated BLAST (blastx). NCBI has a terrific getting-started...gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for expected ORFs..., due to the additional searches involved in translating the nucleotide sequence into all six possible...choose for your sequencing result and can easily translate it, we recommend using blastp over blastx. The...

...visualize cell cycle dynamics, titrate inducible transgene expression, or a variety of other clever applications...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

...cases being reported (right, red), likely due to quarantining {% video_player "embed_player" overrideable...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...imposed on scientists. Figure 6: Model fit. Orange line depicts observed data. Blue line is the sum...depending on when we believe the China COVID-19 date range is, they could be the most impacted or least impacted...

...expressing neurons and GFP (green) to label neurons transduced by each serotype. Working at Addgene has ...answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–response.../10.1038/s41434-018-0028-5 de Backer, M. W. A., Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan...

...antibiotic resistance using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have... joining machinery, so a CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB...diverse, and each phage has a narrow bacterial host range. To further potential clinical applications, Ando...Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe Barrangou. “A CRISPR design for next-generation antimicrobials...

... elaborately pranking each other, dressing in costume, and holding competitions (ranging from cook-offs...orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light work”..., evite, posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of...

...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our...

...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for your... reactions are performed, the next step is to transform competent E. coli cells and select the positive...Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley...

...particles that can transduce cells. Infectious titers are typically quantified by cell transduction assays. Wild-type....qPCR...Digital droplet PCR...DNA dot blot...Transduction assay...SDS-PAGE...Electron microscopy… Any ...titer of a viral vector stock can vary based on transduction target and can also be altered by freeze-thaw...

... package stays in transit for a certain number of days and tracking average transit time for each courier... often weren’t easily accessible, due to a wide range of logistical barriers. Addgene worked to identify...Protocols Agarose Gel Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol...