We narrowed to 801 results for: DES

...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...

... a receptor for PHP.B. Read on to learn how the design of PHP.B may have contributed to this mouse-specific...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...

...updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is... may be unintentionally mutated along with the desired target locus. The PAM sequence is of particular...

...staining. Antigen-containing samples often include undesirable molecules that may bind non-specifically to assay...specific than other methods. In addition, the assay includes both primary and secondary incubation steps, increasing...

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

...polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic acids with high sensitivity...per second. A quantifiable fluorescent signal designates the presence of your DNA of interest, in this...

... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead...Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility caused...

...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...molecular and microbiology for the last two decades as a staff scientist, but before going to graduate ...

...map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been...symbol of opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas...

..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab has shown some...

...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the multiple...

... live cells in real time. Previously, we have described a number of practical considerations that should... herpesvirus.       References 1. Snapp, Erik. "Design and use of fluorescent fusion proteins in cell ...

...antibody-based technique, validation and proper experimental design are essential. In this article, we’ll go through...steps include: counterstaining, mounting sample on slides, and confocal   Figure 2: High-level overview...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...final base editor that was administered to KJ was designated NGC-ABE8e-V106W. The name is hardly flashy, but...

... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...cryo-EM samples from damage at the AWI. Plus, tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim...

...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...one temperature. Two Cas12a-crRNA complexes are designed, each binding to one strand of the reverse-transcribed...

... – including undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name...impossible deadlines, unnecessary disruptions Destabilization – including failure to acknowledge good work...

...single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites, but the insert...the orientation of your insert. You may want to design a diagnostic digest for this purpose. Ideally, ...

... are more involved in the science, experimental design, and execution. Can lead to an independent project...advice Give yourself permission to pursue your own desired path. Careers in science are also not limited to...

... recognition are a few methods by which tumors sidestep immune surveillance: Suppressing Function Antigen...target is never the answer to the multi-level barricades put up by cancer. As mentioned in this article...

...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably... the optogenetic constructs Expression of your desired optogenetic construct in the brain region and cell...

...The recently established case Matter of Dhanasar provides the three-prong test that US Immigration officers.... As Immigration is a federal law, Casseus Law provides legal services to people located in all 50 states...

...guide and use it for quick reference when you're designing your next virus experiment. Subscribe to Viral...

... because the finalists will receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's...

...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...

... research, particularly genetics research, for decades. Its short life cycle, reasonably close genetic...

...and have unleashed them all over our recently redesigned blog. 🐕 Your task is to find them, scavenger...

...over, but signs of holiday spirit still linger (despite the unseasonably warm weather we had last weekend...

... McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also...

...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...

...uncovering ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger...

... An Introduction to Gating in Flow Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling...

...technician, or Principal Investigator. How-to guides for troubleshooting your experiments and getting...

...One of Tom's earliest and most popular videos. Describes fossil rocks and their importance in understanding...

... social media endeavors - in between some much-deserved vacations, sunshine, and beach trips, of course...

...the final four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   ...

...the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes...

... - it's all in the family. 3:56 - 7:04 : Alex describes the MD PhD process and explains why he loved the...

...Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to quantify...

...community and support a wide range of experimental designs. Here are nine great additions, including our first...

...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-...

...Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi and Ben describe their projects 0:28 - 1:12 : Introduction from...

... which results in neutralization or targeted destruction.  Antibodies are composed of two light chains...

...Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench...

...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...

...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...

...that the most cited work in history is a 1951 description of an assay to determine the amount of protein...