We narrowed to 868 results for: REN

...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

...introducing mutations (red nucleotide). Created with BioRender.com. The initial version of EvolvR has an editing...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

... to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized GTPase,...expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS activated...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

...interest. They also “showed that different guide RNAs have different abilities to turn genes on and off..., transformations require electroporation. References & Further Reading: For more information about...

...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth... you're asking of your audience and when things aren't working out, adjust the ask.  I used to do a lot...

...recent positions outside of the lab. Granted, I currently work on the Scientific Support Team here at Addgene...resolves the issue.  If this sounds like about seven different skills to you, you are not wrong. Problem-solving...how you approach new challenges.     Know your strengths! Research experience presents ample opportunities...

...Association for Creatine Deficiencies (ACD) is a parent-let non-profit founded with the mission to support...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular...

...has lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address...proteins that interact with an RNA of interest. Current labeling methods are not ideal for in vivo work...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

...data to prove it is worthless. The OSER assay is currently the best way to test a fluorescent protein’s propensity...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

...Rounding out the remainder, 2% of postdocs who weren’t going to get a salary increase prior to the injunction...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. ...

... plasmid libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can...et al 2016 and Wong et al 2015. Alan Wong is currently an Assistant Professor at the School of Biomedical...systems. He can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy...

...plasmid) and any other important features that differentiate this plasmid from its predecessor. Important...sequencing provider. Each sequencing service will have different requirements for volume and concentration, and...Resources on the Addgene Website View our Plasmid Reference Pages Get Plasmid Backbone Information from Our...

...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...constructs, assemble standard plasmids parts in different combinations, or other new and exciting applications...constructs in a single, high-efficiency step. References: Sequential amplification of cloned DNA as tandem...

...creates strong, well-directed meaning and delivers strength to the sentence. Below are the typical sections...an opportunity to summarize the limitations of current state of the art technologies. Therefore, take ...address some, if not all, of the limitations of current methodologies. This section sets the stage for ...

...for either a circular or linear assembly. If you aren’t constricted to a specific number of fragments, ...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

...compatible with multiple FP(11) fragments, even from a different base FP (for example, EBFP2(1–10) can be used ... useful. Good luck, and happy experimenting! References and Resources Additional resources on the Addgene... Fluorescent Proteins Plasmids and Resources Reference Feng, S., Sekine, S., Pessino, V. et al. (2017...

...a blot, you should not compare the strength of bands from different rounds of detection. Finally, stripping...abundant. If the abundance of your targets isn’t too different, consider antibody affinity: stronger binding ...

...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...great hack for finding relevant papers even if you aren’t familiar with the topic.  Meta Meta is a literature...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...

... distinguish real and artifactual postsynaptic currents (PSCs) this work shows that the artifactual PSCs...changes in TagRFP-T fluorescence intensity at different time points, the authors tracked protein activity... FLIM-FRET acceptors here. See Addgene's FRET reference page for many other common FRET vectors. Murakoshi...

...transactivator, Tat. For more information on the different generations of lentiviral packaging systems please...experiment, it is recommended that you try a few different ratios of transfer, envelope, and packaging plasmids... Addgene’s website for additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic...

...tools for genetic engineering. There are several different viral vectors to choose from, for example gamma-retrovirus...expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...mammalian cells with a single viral infection. References Maetzig T, et al. Gammaretroviral Vectors: Biology...

...publish a new blog post, and live tweet from conferences. Find more tips on using social media as a scientist...communicate science. You can draw live sketches at conferences like @ATJCagan or create agar art with colorful...active with it, new doors started opening up. My current job as a Marie Curie Fellow is a direct consequence...

...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, these...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates, determined...Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim...

...white arrowheads). It is necessary to use two different pairs of target sites for this strategy to work...another. In the field of mouse genetics, FLEx is currently being used to study a wide variety of in vivo ...the genOway press release for more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling...

...workshops, presentations, and lectures. There are currently around 1,000 active makerspaces around the world...learning and communicating with one another.      Currently our main focus is coding the molecular biology...academic labs, we have the capability to study many different mutants because the Caenorhabditis Genetics Center...

...format for events and sessions in scientific conferences. They can result in lively discussions with both...the moderator is not a panelist and will play a different role so her ability to control the event is what...spontaneity so a moderator should be prepared with different questions than those on the prep calls, but often...

...these vectors is controlled by a different promoter resulting in different levels of expression from each...labelled or modified amino acids that are useful in different downstream applications. Cell-free expression ...

...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...blogger, Laura Desrochers! Laura Desrochers is currently a Postdoctoral Scientist at AstraZeneca. She has...

...image provided by Manuel Gonçalves; made with BioRender.   Delivering gRNA and Cas9 using adenoviral...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...Zamyatnin AA Jr (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow...

... the heat. Isolating male and female flies Differentiating male and female flies is essential for setting...their adult phase. It is important to clear out the parent crosses from the container before the progeny begin...thorax to look like stubble (sb). For a better reference of various fly phenotypes, check out the Classical...

...most cases, by using monomeric FPs or FPs from different families, which cannot form heterodimers (i.e....at the National Institutes of Health and he is currently an Associate Professor at Albert Einstein College...avid long distance runner, gardner, and cook.   References 1. Snapp, E.L., et al., Formation of stacked ...

...outcomes can be most effectively achieved with different CRISPR components. Select the appropriate CRISPR...cell lines is quite broad and includes several different methods, so we've summarized some key features...vitro cell culture. The user should review the current literature about their preferred model.  If your...

...expression of Cas9/gRNA and can be used for most of the current genome engineering applications of CRISPR including...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

...Purification Good for antibody-based purification; has inherent enterokinase cleavage site GST Large Protein...protein as pure as possible? Vectors that offer different combinations of tags are readily available, and...information and some protocols can be found in the references provided. Additional resources: Young CL, Britton...

...writer? That’s okay! Your cover letter needs to be coherent and free of typos and grammatical errors. It does...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...experience, having given more than 20 talks at conferences and taught for five years at [name of the university...

... the Howard Hughes Medical Institute. Are you currently on or planning to go on the academic job market...if your model is correct or your data favor a different model. Finding evidence for or against a model...the answer to your research problem. Creating a coherent unified story and model can help make your ideas...

...jarring for the unprepared, and unfortunately, there aren’t a lot of resources out there for scientists making...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...etc.--so everyone’s experience will be a little different. Some of these changes you may enjoy immediately...

... has been crucial for us to keep pace with our current need to analyze nearly 300 sequences each week.... address the need to accelerate science using different approaches. We were also encouraged to see that...pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design, we were free to use multiple...

...remainder of this blog post. Perhaps the biggest difference between Cas13a and Cas9 is that Cas13a binds ...SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus, genotype human DNA, identify...Gearing contributed to the update of this post. References 1. Abudayyeh, Omar O., et al. "C2c2 is a single-component...

...no papers, it's unlikely you'll get a job. The currency of scientific success is publications and grants...to convince me that their search committee is different and needs to be told all of the nuances of the...technologies, i.e. video interviews. For point of reference, I submitted my applications all by snail mail...

... blog post on Easi-CRISPR for more details. References and resources Want to know more? Check out the...Genome Editing General Homologous Recombination References: Repair of Strand Breaks by Homologous Recombination...Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system...

...to a mouse model of muscular dystrophy. Three different splice site donor and acceptor sequences were ...for a transgene to be reconstituted, there is currently no one ideal split vector system. Overall the ...greater and more consistent transgene expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos...

...vegetable! What is an RNA aptamer? RNA is not inherently fluorescent and can be challenging to track intracellularly...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

...Gram-negative bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa...engineering in bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-...for allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...

...Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski Labs now has a new version...control of one of seven enhancers specific for different interneuron populations. The enhancers span all...effort to develop molecular tools for studying different brain cell types. Look for more of these interneuron-targeting...

...therapeutic applications that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib... Does Not Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org...Models Using CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org...

...Fast forward a few hundred years and there are currently about 5.5 million articles in the English version...a team is a really good way of creating and strengthening those social bonds. So never mind how much better... you find there’s a need for an expertise your current team members don’t have - sometimes you just don...

... in the comments section below! Currently, there are over 20 different varieties of PA-FPs available to...function with extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz...