We narrowed to 745 results for: TRI

1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ... CFP set. mTFP1 can be used as a FRET donor to mCitrine and mOrange or for live cell imaging of fusions...

2. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

   Type

   Blog Post

   Published

   Nov. 8, 2018, 3:47 p.m.

   ...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...

3. CRISPR Challenges: Standardization and Homology Directed Repair

   Type

   Blog Post

   Published

   Feb. 14, 2018, 2:36 p.m.

   ...to me. But coming to #KSgenome and seeing the intricacies of DNA repair has emphasized how essential these...

4. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ...downstream applications (Berns, 1972). Using qPCR and restriction enzyme based methods, we showed that the DNA ...

5. Summer SciComm Series: Master of SciComm

   Type

   Blog Post

   Published

   July 21, 2022, 1:15 p.m.

   ...importance of science communication, although the detritus of misinformation in the wake of a global pandemic...

6. RUBY-Red Siliques

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   Blog Post

   Published

   March 1, 2022, 2:15 p.m.

   ...decrease the routine usage of antibiotics which may contribute to the emergence of antibiotic resistant disease...

7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   Published

   June 22, 2021, 1:15 p.m.

   ...to expand upon the work and continue to make contributions towards this COVID-19 pandemic,” says Balazs...

8. Science Careers: Unruly Interests Feed Many Paths

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   Blog Post

   Published

   Sept. 26, 2017, 12:12 p.m.

   ...This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...

9. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   Blog Post

   Published

   Aug. 31, 2023, 1 p.m.

   ...manipulate responses through neurological means like electric stimulation, drug administration, and optogenetics...

10. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ...gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the world, and the question...

11. Don’t Wait To Launch Your Health Science Startup

    Type

    Blog Post

    Published

    Aug. 25, 2015, 2:30 p.m.

    ...quite honestly, does not value your potential contribution to the healthcare landscape.  You will find ...

12. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...29(1):33-41. PubMed. Protein transduction: unrestricted delivery into all cells? Schwarze SR, Hruska...

13. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...This post was contributed by Adam Chin-Fatt, a Ph.D. student at the University of Western Ontario. Adam...

14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type

    Blog Post

    Published

    March 6, 2014, 7:12 p.m.

    ...Mentee reach goals. One of the most important contributions made by a Mentor is to help the Mentee feel ...

15. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ...the serotypes, since these 20 ul samples are distributed individually. See our Serotype Testing AAV inventory...

16. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    Published

    Aug. 2, 2018, 12:56 p.m.

    ...This guest post was contributed by Ally Huang is a 4th year PhD student at MIT. While I had always enjoyed...

17. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...specific for double strand DNA (as compared to spectrophotometric methods that measure all nucleic acids). ...

18. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    Published

    March 19, 2020, 1:15 p.m.

    .... If you’ve used Addgene’s AAV tools, you can contribute data to the AAV Data Hub to help the research...

19. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    Published

    Aug. 23, 2022, 1:15 p.m.

    ...,” the catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance...

20. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type

    Blog Post

    Published

    June 23, 2022, 1:15 p.m.

    ...treatments are currently being tested in clinical trials. It is speeding the process of the discovery/development...

21. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type

    Blog Post

    Published

    April 7, 2022, 1:15 p.m.

    ...variety of roles in synaptic communication and can trigger other forms of neuronal signaling and regulation...

22. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser...

23. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....

24. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ...This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc. Plasmids and PCR...

25. Science Career Options

    Type

    Blog Post

    Published

    Feb. 23, 2017, 1:30 p.m.

    ...in-depth research is applicable to a wide range of industries and is a highly desirable quality sought after...

26. Trading Spaces - Visiting Scientist Edition

    Type

    Blog Post

    Published

    Aug. 22, 2023, 1 p.m.

    ...rights Do you expect to generate data that will contribute to a publication? A grant? A patent? It is never...

27. K. phaffii: Rising to the Occasion

    Type

    Blog Post

    Published

    April 4, 2023, 1 p.m.

    ...recombinant proteins by the pharmaceutical and biotech industries as a protein expression workhorse. This yeast...

28. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    Published

    March 30, 2023, 1:15 p.m.

    ...differentiation To understand how each human TF can contribute to differentiation, the Zhang lab transduced ...

29. Hot Plasmids - January 2023

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    Blog Post

    Published

    Jan. 31, 2023, 2:15 p.m.

    ... Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting by ...

30. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type

    Blog Post

    Published

    April 28, 2021, 1:15 p.m.

    ...grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique": This...

31. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type

    Blog Post

    Published

    Nov. 20, 2018, 8:17 p.m.

    ...when citing, you can copy and paste the text attributing the PI who generated the plasmid, followed by...

32. How to Lead a Great Meeting

    Type

    Blog Post

    Published

    Feb. 9, 2016, 3:30 p.m.

    ...relevant people. How often do you need to meet? Use trial and error to determine the best frequency for your...

33. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    Published

    Aug. 13, 2015, 2:30 p.m.

    ...libraries for use in genetic screens and has contributed much of his knowledge to the CRISPR resources...

34. Management for Scientists: Delegating is Key

    Type

    Blog Post

    Published

    Feb. 20, 2015, 8:30 p.m.

    ...is often dramatically improved when tasks are distributed appropriately. If team members have opportunities...

35. Resume Writing for Non-academic Science Careers

    Type

    Blog Post

    Published

    Dec. 18, 2014, 4:06 p.m.

    ...This post was contributed by Theresa Liao of the University of British Columbia. When transitioning from...

36. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ...This post was contributed by Kurt Thorn of the Nikon Imaging Center at UCSF. A common requirement for...

37. Finding and Joining Your Dream Lab

    Type

    Blog Post

    Published

    April 8, 2014, 2:50 p.m.

    ...working for one person and with a group who all strive for a common big-picture goal. It is worth doing...

38. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    Published

    March 25, 2014, 3:15 p.m.

    ... Marcy Patrick...

39. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...

40. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...within a defined region of a genome. Require stringent bacterial growth conditions. Expression of an ...

41. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...activity. This property makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively...

42. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...translation. Many thanks to Nicole Zurcher who contributed to the writing of this article. Additional Resources...

43. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    Published

    Jan. 21, 2025, 2:15 p.m.

    ...witnessed several colleagues do this while changing industries and positions. Some people discover they truly...

44. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ..., so it no longer overlaps with the control. In tricky experiments, these populations may overlap slightly...

45. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...structural protein of the retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol ...

46. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager...

47. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...selection can take the form of varied temperature, nutrient availability, drugs, and much more.   Workflow...

48. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...edition. New York: Garland Science; 2001. The distribution and functions of immunoglobulin isotypes. Available...

49. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...with dozens of sensors developed for it. Calcium triggers muscle contraction, and calcium channels are activated...

50. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...efficiency. However, gamma-retrovirus infection is restricted to dividing cells only, so is not suitable for...