... Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development, ...GenBank, but it was useful to present it all in one place to get a sense of the similarities and differences...

... and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these...reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the...gene expression.     The LR Reaction takes place between the attL sites of the generated entry clone...

...science. Facebook is continuing its growth, and while many publishers and researchers have Facebook pages...article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science, video...

...overexpressing mice spent more time in the risky bright place, driven by their motivation to explore novel environments...treatment in rodents. In this test, an animal is placed into a tank of water with no support from below...attempts to escape, or passive floating on the water surface. The latter reflects a state of behavioral despair...

...recently moved to our brand new location, with more space to grow and help scientists share resources in new...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the...actually clump together forming one patch on the surface, giving the embryo a “spiny and prickly look” that...

...frequently why many people get into science in the first place. The feeling of camaraderie one gets from a well-functioning...could dread these interactions, but you could also embrace them. Talking about science to non-scientists can...curiosity that led you into science in the first place. Finally, if you still find the idea of not holding...

...on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an ...-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in performance...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...

...focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA...RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a...is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought together a strong...

...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...fluorescent proteins might not localize to the right places, which can provide misleading information on where...

...conference can be intimidating. And it can be exacerbated when everyone seems to know one another, but ...program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring circles We looked at...this first year. Mentors Amanda Gunn, Bryan Gibb, Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton...

...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

...protein attaches to cell surface receptors, other important binding events take place during the course of...viral envelop and the receptors on the target cell surface. The possible interactions and hence possible targets...

...Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...activated patterning of dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27...

...target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test can...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...

...displayed on the surface of a cell of interest, where it can label interacting cell surface proteins with...capable of promiscuously labeling various cell surface proteins that contain a monoglycine residue at ...

...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of... for an “escape” virus to propagate and infect adjacent cells. It will be important to determine if CRISPR...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...

... characterised antibodies against all human extracellular proteins with community-based validation. They...experiments on similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000...their experimental designs. There are many more facets to reproducibility issues in the biological sciences...

... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...GABA neuron, for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more...

...you want to know if they co-localize at the same place in the cell? Or you know that the presence of several...your microscope. Abcam's sample protocol is a good place to get started.  One important consideration when...the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split Channels...

...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...GAG), and are (typically) tethered to the cell surface by a GPI-anchor. Image courtesy of IPI.   ...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...