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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 610 results for: ACE;

Showing: 241 - 260 of 610 results

  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...formation of disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose...

  2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter ...in the molecular crevices proteins on the cell surface. Figure 1: Comparison of common antibody,... is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain folded...

  3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...definitive, but visual identification is a good place to start before taking on more time consuming and...fitness cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using...Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine with hydrophilic...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type...produces the phenotype you screened for in the first place. With the proper experimental design and validation...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... who need to overexpress and purify proteins can face any number of technical challenges depending on ...protease. A TEV protease cleavage site is frequently placed between two tags being used for two rounds of purification...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an ...-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in performance...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...science. Facebook is continuing its growth, and while many publishers and researchers have Facebook pages...article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science, video...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...protein attaches to cell surface receptors, other important binding events take place during the course of...viral envelop and the receptors on the target cell surface. The possible interactions and hence possible targets...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...fluorescent proteins might not localize to the right places, which can provide misleading information on where...

  11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...conference can be intimidating. And it can be exacerbated when everyone seems to know one another, but ...program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring circles We looked at...this first year. Mentors Amanda Gunn, Bryan Gibb, Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...overexpressing mice spent more time in the risky bright place, driven by their motivation to explore novel environments...treatment in rodents. In this test, an animal is placed into a tank of water with no support from below...attempts to escape, or passive floating on the water surface. The latter reflects a state of behavioral despair...

  13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...recently moved to our brand new location, with more space to grow and help scientists share resources in new...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the...actually clump together forming one patch on the surface, giving the embryo a “spiny and prickly look” that...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...frequently why many people get into science in the first place. The feeling of camaraderie one gets from a well-functioning...could dread these interactions, but you could also embrace them. Talking about science to non-scientists can...curiosity that led you into science in the first place. Finally, if you still find the idea of not holding...

  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA...RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a...is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought together a strong...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development, ...GenBank, but it was useful to present it all in one place to get a sense of the similarities and differences...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these...reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the...gene expression.     The LR Reaction takes place between the attL sites of the generated entry clone...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of... for an “escape” virus to propagate and infect adjacent cells. It will be important to determine if CRISPR...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...

  19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test can...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...
Showing: 241 - 260 of 610 results