...variations in gene expression, they found that for approximately 40% of the identified genes, the genes were ...lines from individuals of African descent have approximately 20% more single nucleotide polymorphisms (SNPs...ancestry-associated cancer dependencies are undercounted by approximately 20%. In some cases, this may lead researchers...map. Now, any data that came from CRISPR guides mapped to a SNP would be ignored. Working with Lia Petronio...8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, ...

...in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first gene therapy for the treatment...AAV-based gene therapy treatment for a genetic disease approved by the FDA is for a rare eye disease and not another...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...medicine. References and further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

...lot of activities (try for a dozen!). The best approach may not be “let’s try to make this STEM activity...that would compliment the STEM curriculum. As it happened, I’d met some of the 8th grade science teachers...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....analogy that involves student participation, make it happen! How should we use CRISPR? How shouldn’t we use... I came out of the experience with a renewed appreciation of our science teachers’ difficult-but-rewarding...

...In this Plasmids 101 post, we’ll cover why this happens, how it might affect your work, and how understanding...rely on chance (or random diffusion) to make this happen.  There are several different versions of the plasmid...way to bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids...pestis using plasmid incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111...Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural Biology...

...connection you made at the job fair when you formally apply for the position. This post on Business Insider ...re likely to find current job openings. A good approach is to do this first for companies at which you...connection. That way, once you’ve identified an appropriate job opening, you can send your contact an email...the assistance of recruiters for identifying appropriate job candidates. If you do receive such a call...those relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible...

...move from lab to office will likely happen eventually. If you approach that transition dreading the change... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...thinking” in the position to which she was applying. I probably did not respond to her question as well then...adjustment period will be that much longer. If you approach the transition embracing the opportunity for growth...

...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop... tools we make available. This prioritization happens for good reasons. We began offering our viral vector...remains the more requested option, and jGCaMP8 appears to be growing in requests faster than jGCaMP7 (...viral vectors. This process builds on our former approach and involves reading primary literature, soliciting...redesigned Data Hub in 2022. It’s influenced the way we approach our viral vector educational resources, from eBooks...

...contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp...website invites you to input the target region (approximately 500bp is best) and select the organism. It will...different types of gRNA design software. When you are happy with your selection, ordering the sgRNA is simple...process, you should first develop primers to the approximately 500 base pair sequence surrounding your gRNA...

... tangible and applicable to your situation.   Thinking Outside the Box: Team Approaches At some point ...required to develop new projects and test novel approaches, a necessary skill set for any scientist. In ...lab   Thinking Outside the Box: Individual Approaches Whether it’s finding the source of contamination...creative skills you have developed in the lab can be applied anywhere! References and Resources References ...

...to generate new pEcgRNA. pEcCas/pEcgRNA can be applied not only in coli K-12 strains, but also in E. coli...genome editing in microorganisms which could be applied to optimization of genome editing tools.   Jieze...engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of...research focuses mainly on the development and application of a multiplex genome editing system based on...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org...

...exist in the real world and corrective approaches and appropriate controls are required to make this method... this technique has revolutionised the way we apprehend molecular complexes and is still a very useful...experiment. You can then choose the most efficient approach for your own system. The simplest and the most...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

...one of two ways - each junction resolution could happen on the crossing strand (horizontally at the purple...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)... PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS...

...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...They generated variants optimized for different applications (v82 has higher affinity for glutamate but slower... in the fluorescent properties, pYtags are an appealing tool for monitoring RTK signaling.  Figure 4:...lower binding affinities to broaden the range of applications. Image reproduced from Torres Cabán et al. 2022...screening platform developed here is also a promising approach for developing sensors for other targets. Figure...

...molecular biology applications for biotin and avidin. A short list of some other common applications and links...downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications including... feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application of avidin-biotin technology to improve cell adhesion...

... talks and how to make them serve you well. The happy byproduct might just be a career opportunity. The...non-academic settings. Many of these guidelines will apply to any type of science presentation, but, for more...and venue before tailoring each presentation appropriately.  Find out who will be listening.  Are they ...goal is to get the listeners to be thinking “What happens next?”  Describe how you pushed to a solution or... use no notes and can concentrate on delivery, rapport with the audience, and their own excitement about...

...sell them. Types of Conflict Before explaining my approach to conflict resolution, it’s important to recognize...day-to-day operations and keeping our customers happy, but inevitably there will be gray areas that either...you can help everyone walk away from a conflict happy, feeling they each got enough of what they wanted...Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based on various levels...yourself gravitating most frequently towards these approaches, you may need to put more conscious effort into...

...ways to clone DNA fragments which have provided appealing alternatives to restriction enzyme cloning. These... amplification with primers that contain the appropriate homologous sequences. NEB recommends an overlap...sequences remaining in the final product. The long overlapping region between fragments also better ensures ... of the fragments as compared to the smaller overlapping sequences left with a restriction digest. A study...cloning kits available which are all based on long-overlapping regions, have no requirements for restriction...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place....org with any thoughts, questions, or feedback. Happy reading! ...

...it got “dirty”, with multiple sequence traces overlapping each other! When the count was done, 75% of the...but not least, when using CRISPR, remember to appreciate the fact that it was first discovered in an obscure... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-...

...blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs...capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya et al., 2016). In this case, location...location and sequence are of approximately equal importance in design – an optimized sequence will do ... using knockout or transcriptional modulation approaches. Avoiding off-target effects The off-target ...fully predict which sites will and will not show appreciable levels of modification. Whole-genome sequencing...