We narrowed to 256 results for: Alk

1. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence of Off-Target...

2. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ...declare your bench “RNase free” and refrain from talking to friends and colleagues while working with these...

3. Building Global Connections with the International Mentorship Program USA-EUROPE

   Type

   Blog Post

   Published

   Nov. 6, 2015, 3:30 p.m.

   ...Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska Institutet, among ...

4. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks...

5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

   Type

   Blog Post

   Published

   March 7, 2017, 3:30 p.m.

   ...that are essential for experiments but that nobody talks about. After months of troubleshooting, those people...

6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

   Type

   Blog Post

   Published

   March 21, 2024, 1:15 p.m.

   ...dyes with different emission spectra to avoid crosstalk/crossover to reduce ‘muddying’ of the signal and...

7. Custom CRISPR Screens & the Green Listed Software

   Type

   Blog Post

   Published

   July 11, 2017, 2:30 p.m.

   ... interest. Selected pathways  I attended a great talk when I was a PhD student. In particular, one slide...

8. Special Delivery: Fluorophore Targeting for FRET Studies

   Type

   Blog Post

   Published

   July 19, 2016, 2:30 p.m.

   ...s best to consider these cases separately when talking about the tools that are available. Let’s consider...

9. A Primer on Optogenetics: Introduction and Opsin Delivery

   Type

   Blog Post

   Published

   Sept. 10, 2015, 2:30 p.m.

   ...the intended brain region and/or cell type (I’ll talk more about this in detail later). But what are the...

10. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ... and yes those purification columns we’ve been talking about!) are a significant source of waste in laboratories...

11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    .... K., Roberts, N., Zhang, L., Vakulskas, C. A., Walker, J. A., Kadina, A. P., Zepeda, A. E., Holden, K...

12. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ... if you’re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is ...

13. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...nucleoli, and little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins,...

14. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...doi.org/10.1093/bioinformatics/btr065Freeman WM; Walker SJ; Vrana KE. 1999. Quantitative RT-PCR: pitfalls...

15. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...robust initiation of reprogramming (evaluated by an alkaline phosphatase-positive assay) as well as stabilization...

16. Troubleshooting and Optimizing a Western Blot

    Type

    Blog Post

    Published

    Sept. 17, 2024, 1:15 p.m.

    ..., you’re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting...