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We narrowed to 523 results for: Cit

Showing: 241 - 260 of 523 results
  1. Hot Plasmids: May 2026

    Type
    Blog Post
    Published
    May 5, 2026, 1:15 p.m.
    ...Isotype Control from IPI By Ashley Waldron We are excited to now offer a rabbit IgG isotype control made ...antigen-presenting cell screening. TCRAFT also allows antigen specificity to be linked to gene expression data. In validating...
  2. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story...
  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...depends on the ability to monitor their signals – electricity! The research tools for studying voltage within...as neuron subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic...
  4. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...done the research, gathered up your data into an exciting story, and are ready to present your findings ...person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch throughout...
  5. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...Ethiopian?) to where you are going on vacation (New York City or Hawaii?). So how can you approach improving this...starting date with the company so they know I’m excited while still being able to take my vacation? I want...
  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express... Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new eBook...
  7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...where limited expression of Cas9 is required and specificity is a concern, such as knockout generation or ... in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein in the host...
  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...
  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...of concentrations but suffers from decreased specificity, especially as it approaches the minimum and ...are more disheartening than seeing a potentially exciting result quashed by a bad standard curve. Take the...
  10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...efficiencies of 90-100% (Fig. 2B), and >99% on-target specificity. Additionally, by expressing multiple crRNAs ...the CRISPR toolbox RNA-guided transposases are exciting new additions to the growing CRISPR toolbox for...
  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular... mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable...
  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...for applications that require the large genetic capacity of a lentiviral vector but for which transient...
  13. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression of AAV...
  14. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...
  15. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...development, transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC...
  16. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...culture. Our developers are a diverse group of ethnicities, cultures, genders, educational, and professional...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...
  17. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... make your ideas easier to understand and more exciting. Practice presenting your chalk talk Perfect your...research program. You should be proud of it and be excited about getting the opportunity to realize your vision...
  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...nonprofits, who tend to prioritize candidates that are excited about their work and mission.  How do I know what...you excel in the role. It can also convey your excitement for that particular job, your commitment to the...
Showing: 241 - 260 of 523 results