We narrowed to 372 results for: ENA;

1. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   Published

   March 23, 2017, 2:30 p.m.

   ...upgrades to our inventory management systems to enable efficient, tracked production and distribution ...

2. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ... various serotypes available (AAV2, AAV5, AAV8) enable different patterns of transduction and transgene...

3. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

4. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...

5. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   Published

   Feb. 21, 2019, 1:15 p.m.

   ...Shakked O., et al. "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable...

6. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...a virus can infect. The VSV-G envelope protein enables this virus to infect many different cell types ...

7. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...biological fields the number potential of experiments enabled by physical, software, and biology-based tools ...

8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA...

9. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

10. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back...

11. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split...

12. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson...

13. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

14. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ... that template for repair. By using these two scenarios, you can either knock out a gene or knock in a...

15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic...

16. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...antigen tend to work very well for applications that denature a protein to its primary (linear) structure using...

17. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...

18. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein...

19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further ...

20. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome...