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Showing: 241 - 260 of 520 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...ever both to engineer specific DNA edits and to perform functional screens to identify genes involved in...chromatin status). No computational prediction is ever perfect, but this can decrease the number of guides one...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s emission peak...higher the emission from the acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...polymerase is a phage-derived polymerase that can perform whole genome amplification (by a mechanism known...below). This initial transformation should be performed using a highly competent strain that you can use...

  4. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...extremely bright, then the noise likely will not interfere. If the fluorescence from your target is weak,...fluorescence of the sample, or autofluorescence, also interferes with data interpretation and can be addressed...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...more.  Conclusion   Optogenetic tools provide a powerful way to study multiple aspects of cellular activity...important to understand the details of how each tool performs and the consequences of off-target activity. Whether...

  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...produce completely transgenic offspring. Although powerful, this technology is cumbersome to use, not that...concept, straightforward to use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired...

  7. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... questions one could pursue and experiments to perform. It is tempting to pursue a new line of inquiry... school student. Learn to teach how to design, perform, and interpret experiments. You'll be better prepared...

  8. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...for guiding new deposits into the repository, performing quality control, and providing technical customer...strong sense of purpose, an excellent leader, and perfectly clear lines of communication, but if teammates...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...link genotype and phenotype. It is also key to perform these validations as translational researchers ...the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse ...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...these types of experiments easier and faster to perform. Introduction to flow cytometry Flow cytometry ...containing a negative cell is discarded. FACS can be performed aseptically allowing users to culture the cells...

  12. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...hyper-competitive environment where getting hired hinges on performing near miracles. When evidence does not affirm ...in the picture) and this march proved to be the perfect opportunity to cement in my children's heads the...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual... tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

  17. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system was coined...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. Finally,...to purify the reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization...

  19. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...the results are correct. Primers and probes can interfere with amplification reactions, and reagents can...

  20. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...different than the validation assays, we recommend performing your own validation assays. If you’re using previously...interest is not available in a pure form, you can perform relative quantification, which measures protein...
Showing: 241 - 260 of 520 results