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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 530 results for: Erf

Showing: 241 - 260 of 530 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...on the fly”. This becomes intuitive with touch interfaces where the viewer is more likely to want to manipulate... to Research Data? - by Francine Berman and Vint Cerf (Science, August 2013) PLOS’ Bold Data Policy - ...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...time-consuming purification processes, and are powerful, due to their ability to integrate into the host...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... can also be easily knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing... Andrew, et al. "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...molecular processes that make aptamer biology a powerful tool. In this post, we’ll take a deeper dive into... Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide...

  7. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...extremely bright, then the noise likely will not interfere. If the fluorescence from your target is weak,...fluorescence of the sample, or autofluorescence, also interferes with data interpretation and can be addressed...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...more.  Conclusion   Optogenetic tools provide a powerful way to study multiple aspects of cellular activity...important to understand the details of how each tool performs and the consequences of off-target activity. Whether...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's toolkit is the empty plasmid...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...ever both to engineer specific DNA edits and to perform functional screens to identify genes involved in...chromatin status). No computational prediction is ever perfect, but this can decrease the number of guides one...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s emission peak...higher the emission from the acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...polymerase is a phage-derived polymerase that can perform whole genome amplification (by a mechanism known...below). This initial transformation should be performed using a highly competent strain that you can use...

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...produce completely transgenic offspring. Although powerful, this technology is cumbersome to use, not that...concept, straightforward to use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired...

  14. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...for guiding new deposits into the repository, performing quality control, and providing technical customer...strong sense of purpose, an excellent leader, and perfectly clear lines of communication, but if teammates...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...link genotype and phenotype. It is also key to perform these validations as translational researchers ...the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse ...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... questions one could pursue and experiments to perform. It is tempting to pursue a new line of inquiry... school student. Learn to teach how to design, perform, and interpret experiments. You'll be better prepared...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...these types of experiments easier and faster to perform. Introduction to flow cytometry Flow cytometry ...containing a negative cell is discarded. FACS can be performed aseptically allowing users to culture the cells...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...hyper-competitive environment where getting hired hinges on performing near miracles. When evidence does not affirm ...in the picture) and this march proved to be the perfect opportunity to cement in my children's heads the...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual... tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of...
Showing: 241 - 260 of 530 results