We narrowed to 263 results for: INO

1. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ...proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the...

2. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ... from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848-57. PubMed...

3. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ..., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S...

4. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   Published

   June 1, 2023, 1:15 p.m.

   ...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

5. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ...doi.org/10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An ...

6. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry...

7. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ... loading. Denature the samples to their primary amino acid sequence by boiling in the presence of a reducing...

8. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...pair changes as would be the case with ssODNs. One minor drawback with using PBx for excision is its efficiency...

9. A Guide to Getting Started in Undergrad Research

   Type

   Blog Post

   Published

   June 8, 2021, 1:15 p.m.

   ...generation students, women, BIPOC, and other minoritized populations. Research universities often have...

10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...target. The (T)TTN PAM motif required by the Acidaminococcus sp. BV3L6 Cas12a (AsCpf1) eases this constraint...

11. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...order to aid viral binding. This method, called spinoculation, has been found very effective for T cells transduction...

12. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...ultraviolet (UV) light, they do so via aromatic amino acids found in all proteins, so you can't distinguish...

13. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...PubMed Central PMCID: PMC4559080. 5. Tzelepis, Konstantinos, et al. "A CRISPR dropout screen identifies ...

14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...’s excitation spectrum- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated...

15. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ... recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced ...

16. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...standard mutation notation. Please indicate the amino acid change as well as any functional consequences...

17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...base editors have the potential to correct only a minority of those variants in most cell types. But prime...

18. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...comments below! References  1. Sano, Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel...

19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...learn more about site-specific incorporation of amino acids using pyrrolysl tRNA synthetase, read this...

20. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...reprogramming discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4 isoform (KLF4s...