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We narrowed to 821 results for: LAS

Showing: 241 - 260 of 821 results

  1. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...your favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.  Download...background.         If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds ...

  2. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...edits in protoplasts or plants of rice and wheat. Read the paper in Genome Biology Find plasmids containing...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...paper in Nature Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine deaminase...Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA cloning (STAgR) String ...the paper in PLoS One Find the STAgR cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in...Gross’s lab recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids Topics: CRISPR, Other CRISPR... where we highlight a few of the newest CRISPR plasmids available at Addgene. If you want to read more...will cover: Miniature CRISPR-Cas systems SEND plasmids Prime editing improvements Miniature CRISPR-Cas...Read the paper in Molecular Cell Find the CasMINI plasmids Efficient CRISPR editing with a hypercompact ...paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f...cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in... in Nature Chemical Biology Find the AsCas12f1 plasmids   Selective endogenous encapsidation for cellular...

  4. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... As reviewed in our Plasmids 101 series, proteins are overexpressed from a plasmid construct, most often...multiplexing. pCri plasmids are divided into two sets, each of which includes plasmids with different varieties...on the Addgene Blog Plasmids 101: E. coli strains for protein expression Plasmids 101: Protein tags ...varieties of tags, both N- and C-terminal. Each set of plasmids uses the same restriction enzymes for cloning ...for one protein quickly and easily. These tagged plasmids also include cleavage sites should one need to...other tags used in the pCri system, please see Plasmids 101: Protein Tags. Beyond E. coli: purifying ...subtilis and Pichia pastoris pCri also includes plasmids designed for protein expression in systems other...

  5. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... by 10% plasma, but GeoCas9 remained active in up to 30% plasma. Figure 2: Human plasma longevity...activity in HEK293’s. GeoCas9 stability in human plasma The last trick GeoCas9 has up its sleeve is its increased...diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human plasma. The discovery...incubated SpCas9 and GeoCas9 RNPs in diluted human plasma for 8 hours at 37°C. Substrate was then added and... SpCas9 or GeoCas9 RNPs were incubated in human plasma diluted with reaction buffer for 8 hours at either...excited about? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let us know...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...

  6. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...three plasmids — a transfer plasmid containing your gene of interest plus two packaging plasmids (three...baculovirus plasmid. Production of baculovirus requires only two plasmids — your transfer plasmid and the ...three packaging plasmids for 3rd generation lentivirus production). The packaging plasmids are widely available...to worry about cloning your transfer plasmid. The transfer plasmids have different packaging capacities...called a bacmid. Like AAV and lentiviral packaging plasmids, bacmids are readily available, so the amount ...insect and mammalian systems. Baculovirus transfer plasmids have a much larger packaging capacity — up to ...can produce AAV and lentivirus if needed # of plasmids Three or four Two Packaging limit ~4.7 kb (AAV...

  7. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...features was our collection of popular plasmid sequences. These plasmids contain features such as antibiotic... our SnapGene powered plasmid maps Learn how we use SnapGene during the plasmid quality control process...out full plasmid sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis ...design was done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent...popular aspects - its ability to annotate a raw plasmid sequence and display frequently used genes and ...when the DNA sequence has changed. Identifying plasmid control elements Coding sequence features are relatively... boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly wrong....

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn...H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV...AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...genome is lethal. If this DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which...sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, ...genes in E. coli using plasmid and phagemid delivery systems. Phagemids are plasmids containing a phage origin...sequence results in cell death. Plasmid targeting may result in plasmid loss and antibiotic resensitization...this resistance gene being encoded by a high-copy plasmid. In the case of the genomically encoded quinolone...RNAs (crRNAs) targeting either genomic (blue) or plasmid-based sequences (red). Treating a mixed population...specifically targets bacteria with the given genomic or plasmid sequence from a mixed population without affecting...

  10. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...Best-in-class collection at Addgene The GTA contains data on thousands of AAV enhancer plasmids, which ... the Addgene blog Plasmids 101: Gene, enhancer, and promoter trapping Plasmids 101: The Promoter Region...has culminated in a collection of AAV enhancer plasmids with high specificity and brightness, now available...Adapted from the Allen Institute's Genetic Tools Atlas. To evaluate the resulting expression of the...available at the Allen Institute’s Genetic Tools Atlas (GTA). The GTA has a wealth of information and includes...partnered with the AIBS to showcase the “best-in-class” enhancers. The Allen Institute for Brain Science...including the Allen Institute for Cell Science Plasmid Collection and the BRAIN Armamentarium Collection...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... that made it easy to share plasmids, both for people depositing plasmids and for people requesting them... Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression...Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your... Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid? How to Design...video, however, is “What is a Plasmid?”, An animated explainer of a plasmid and its parts that helps the...them. Prior to this, many plasmids were available, but they often weren’t easily accessible, due to a ...barriers, allowing people to access a wide catalog of plasmids from all over the world. This idea, that available...

  12. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ... after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your...with 210 plasmids from the collection. You can find the full list here. Most of these plasmids are empty...cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest...you can focus on what you want to do with your plasmid without spending time and resources in preparative...research and discovery.” Here are the details. Plasmids offered as cloning grade DNA As we begin testing...molecular cloning. Plus, we decided to include 10 plasmids that can be directly used in the research on the... the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists. This collection...

  13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...of the second plasmid can be done in one day in one assembly reaction. This second plasmid saves you a ... page went up last month, over 340 people have signed up to receive alerts when plasmids become available...and Myers for speaking with us! Find the Plasmids: Plasmids for protein tagging with CRISPR Additional... in many cases. In an effort to track down every last one of those gene regulatory proteins and the places... available at Addgene. For more details on the plasmids that are currently available along with detailed...system, what we are doing is adding in a second plasmid to target an epitope tag onto the transcription...second vector is a homology directed repair donor plasmid with homology arms and a selection marker. If things...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID along...undergoes a conformational change and binds the plasma membrane under blue light CRY2-CIB1 heterodimerizes...impossible to target to specific subcellular regions, laser ablation can damage other cellular structures, and...genetic mutations are not easily reversible. Late last year, the Akhmanova Lab published a new optogenetic...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous publications, they described...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...

  15. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ... some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...start a new decade, we’re also looking back at the last year of scientific discovery. We’ve documented many...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene..., and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...

  16. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...collection of plasmids each carrying a single gene with a defined mutation or gene fusion. Plasmids carrying...cancer research, like PIK3CA (Plasmid 116771: pHAGE-PIK3CA) and EGFR (Plasmid 116731: pHAGE-EGFR), are represented...Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources ...mutations, and the group has deposited several hundred plasmids from this study with Addgene. Read on to learn...learn more about their work, and how these plasmids can help cancer researchers studying an assortment of...vitro approach. Using data from The Cancer Genome Atlas (TCGA), a catalog of genetic mutations responsible...well. They called this library the CTD2 FASMIC Plasmid Collection for “Functional Annotation of Somatic...

  17. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...of Addgene plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N ...out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology arms and...the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant female mice...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

  18. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...myogenic precursor cells [5]. Classification of stem cells Stem cells are classified into two broad types according... Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ... inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated mostly from abnormal and...C-MYC. iPSCs were first generated from mouse fibroblasts in 2006 in Shinya Yamanaka’s lab, where the reprogramming...successful generation of human iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively...to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble...system for research in regenerative medicine over the last decade. In the next post of this series, we’ll discuss...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...PITCh-gRNA binding sites. This PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, ...Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about... knock-ins, some labs have used NHEJ for whole plasmid integration; however, since NHEJ is error-prone...cloning. An overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and .... The PITCh-gRNA creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific...locus-specific gRNA The gRNA should target near the last coding exon of your gene of interest. For ideal ...

  20. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, so adopting...mail. Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing...services don’t offer plastic recycling, specialized companies can recycle plastic-- you just have to find...other scientists save resources by sharing plasmids Find plasmids at Addgene ...recycling! With the popularity of single-use items, plastic is a substantial portion of lab waste. Despite ...manufacturing, accounting for 40% of total plastic usage (Geyer et al., 2017). While most single-stream or city ...Corning takes back and recycles a lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product...
Showing: 241 - 260 of 821 results