We narrowed to 473 results for: NOL
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TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....also be used with the most cutting-edge imaging technologies such as array tomography, super-resolution STORM...W. Davidson. "Advances in fluorescent protein technology." Journal of cell science 120.24 (2007): 4247...
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Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays for over ...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....necessary), there are opportunities to work for biotechnology companies and help other researchers use the...experience and scientific knowledge they require. Biotechnology companies vary to what extent they hire experts... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m...., Methylophilus methyltrophus, Methylococcus methanolicus, Methylosinus trichosporium, Myxococcus xanthus...protein expression across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https:/... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....discovery through the creation of innovative technologies. To date his group has develop several popular...genome editing and transcription modulation. Nat Biotechnol 33:139–142 . https://doi.org/10.1038/nbt.3149... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....tool used in the fields of oncology, virology, immunology, microbiology, and pharmacology (many of which...stance on the matter. Funding bodies such as the Biotechnology and Biological Sciences Research Council (BBSRC... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....ribonucleoprotein complexes in vivo. Nature Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of scarless mutations...genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): 233. PubMed PMID: 23360965. PubMed... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m..... To indicate the sequence, people often use terminology like “C-terminal fusion,” but I find this very...artifacts. This is an important downside of the technology and should always be considered when interpreting... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m.... as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent Advances on Physiology...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m.... vectors carrying parts of a larger gene. Nat Biotechnol 20:697–701 . https://doi.org/10.1038/nbt0702-...-splicing adeno-associated viral vectors. Nat Biotechnol 23:1435–1439 . https://doi.org/10.1038/nbt1153... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....searches. I've updated chapters to reflect new technologies, i.e. video interviews. For point of reference...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....modifications feasible and even easy. This targeted-break technology coupled with HDR is a powerful site-specific ...principles driving CRISPRs and genome editing technology. Links: Zhang Lab’s CRISPR FAQs CRISPR forum... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....trying to get hands-on lab experience is that the technology is so advanced that most of the procedures in...own challenges. I’ve had to become skilled with technology like Skype in order to have meetings between ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments ...countless other systems, whereas import of CRISPR technology into C. elegans has been a transformative innovation... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R,...Antibody validation: A view from the mountains. New Biotechnol 45:1–8. https://doi.org/10.1016/j.nbt.2018.08.002... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....questions concern great scientific problems, but the technology may not exist to answer the question or it might...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...