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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 241 - 260 of 628 results

  2. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...postdoc Frank Graham’s 293rd experiment, hence the nomenclature we use today: HEK-293. The exact origin of the... option! Especially if your research interests include receptor biology, ion channels, or involve human...

  3. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...month.  We are currently focused on two projects, including one led by a talented biomedical engineering team...still able to get through to a number of experts, including Harvard/MIT faculty, venture capital and angel...

  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...the specificity of their luciferase assay, they concluded that components in the buffer used for protein...works in multiple types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, ...

  5. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit...RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can...

  6. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel valued...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

  7. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... new contact to connect on LinkedIn and always include a personal note about where you met and what you...can be as simple as “it was nice meeting you.” Include follow-up questions or requests for advice based...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype testing jGCaMP7c for...best serotype for their particular study, which includes comparing the transduction patterns of different...

  9. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...pivotal role in an array of biological processes including development, the immune system, and cell turnover...been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that ...

  10. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...navel-gaze over from the last couple of weeks, including whether or not scientists can recognize so-called...work on emerging issues in novel technologies, including self-driving vehicles, synthetic biology, biosecurity...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...libraries. NEB also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ..., across four different types of human cells, including T cells and hematopoietic stem and progenitor ... from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely on an upstream...

  15. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...factors contribute to the immunogenicity of a drug, including the product origin; purity, mechanism, and stability...F. Weinbauer (Eds.), The Nonhuman Primate in Nonclinical Drug Development and Safety Assessment (pp. 519...

  16. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...understand and grow the scientific ecosystem, including understanding how shared materials are used to...ll be confident in what to expect. No system, including MTAs, is perfect, but it does provide consistency...

  18. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery... promising clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...
Showing: 241 - 260 of 628 results