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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 459 results for: PAC;

Showing: 241 - 260 of 459 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

  2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    .... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence.... L., Medema, R. H., & van Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand...

  3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...selection marker to generate stable cell lines. Packaging and Envelope plasmids provide the necessary components...AAV transduction Only compatible with SaCas9 (packaging limit ~4.5kb). CRISPR elements are inserted into...

  4. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...genomic integration. Lab-engineered viruses will package your genetic cargo in lieu of their own genomes...genomes and deposit this cargo upon host infection. Packaging viruses also have various serotypes – the surface...

  5. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...with picking universities?” and “How does COVID-19 impact all of this?” are all very valid questions, and...program will allow for you to have the time and space to delve into what matters to you. It can also be...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...injection titers (1,2), and cell types used for packaging viral particles (6), can influence vector performance...

  9. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...counterparts, they will also be easier to deliver in low-capacity vectors, such as adeno-associated viral (AAV) ...

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    .... Instead we picniced outside of our new office space on the nicely manicured lawn of University Park ... master drywall hanger?  4. Utilize your office space too Balance point number 3 with doing fun things...

  11. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...

  12. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...decided to take a different approach. His own most impactful learning experiences happened in the lab, and ...analyze their recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function...

  13. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...well-documented history of racial bias, which still impacts and influences current research. Understanding ... reflects European ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...from quenching by aqueous solvent. This tightly-packed structure explains the importance of the entire...stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be used, when...

  17. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

  19. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...have developed your research vision, you need to package it as a roughly 30 minute chalk talk. You will ...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos... proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of the crRNA can be customized as well, including...
Showing: 241 - 260 of 459 results