We narrowed to 372 results for: RELA

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...approaches. Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed...

2. Components of CRISPR/Cas9

   Type

   Blog Post

   Published

   Feb. 2, 2016, 5 p.m.

   ...use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome and a small RNA (gRNA) ...

3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ... is sufficient to use a spectrum for a closely related protein, if you know that your protein of interest...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ... present, it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ..., in principle, these experiments could find correlations between variants in a gene of poorly classified...

6. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...certain to choose your recommenders based on your relationships to the person as well as their current sphere...

7. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...control processes. For the many other COVID-19 related plasmids in our collection, we’ve relied on Sanger...

8. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...researchers in creating and sharing knowledge, related to the types of materials we help distribute and...

9. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...double-checking details, or just taking a moment to relax. During really stressful days, I would even take...

10. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...can be very helpful if you're trying to find a relatively rare protein in an experiment with many proteins...

11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is...

12. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ... workhorse adeno associated virus (AAV) shows relatively low retrograde transport efficiency in most serotypes...

13. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...

14. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...their work on the shoulders of giants. The still relatively young movement of sharing open hardware has a...

15. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...cells and typically only in the same or closely related species from which they were derived. Similarly...

16. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...

17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ... a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged...

18. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...closely together on several projects and this is one related to gene expression. One goal of the ENCODE project...

19. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential...

20. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    Published

    May 22, 2014, 4:02 p.m.

    ...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...