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We narrowed to 836 results for: RING

Showing: 241 - 260 of 836 results

  1. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering. 2008;2:5... fluorescent protein were fused in-frame to a tethering partner, which directed localization of the fluorophore...Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm...Constraints on the order of the modules, because neighboring fragments require compatible cohesive ends Engler...biological part assembly. Journal of biological engineering. 2010;4(1):1. Pubmed PMID: 20205762. PubMed Central...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...by loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization... Tillberg, et al. 2016). That being said, the anchoring, digestion, and expansion steps can impact the...occurs, due to a reduction in target concentration during expansion. One group has found that using Fab fragment...et al (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat Biotechnol...

  3. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...are syringe or vacuum infiltration. Syringe infiltration (D'Aoust et al., 2009): Usually a syringe without...contains a plasmid with a T-DNA (transfer-DNA) region. During infection this T-DNA region is transferred into...transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...Transient expression of antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ... You need to balance respectfully answering questions while steering the conversation back to your vision...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...proposal will distinguish between competing models. Comparing model A vs. B is more constructive than testing...vision. On the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors...and consider eating one before your chalk talk. Bring your own bottle of water. If possible, write down...

  5. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...efficiency and thus protein expression without altering the sequence of the protein. As DNA synthesis ...tRNAs, including those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous ...of translation. The ribosome may slow and pause during elongation which may actually be necessary for ... SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-pairing mechanism for translation initiation in eukaryotes...02)00362-x Stadler M, Fire A (2011) Wobble base-pairing slows in vivo translation elongation in metazoans...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...inteins. Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins...Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819–823...Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl..., Grimm D (2014) CRISPR/Cas9-mediated genome engineering: An adeno-associated viral (AAV) vector toolbox...Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat... Mary Gearing...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...But CRBN has been difficult to study in vitro, requiring either expressing it with additional cofactors...faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also... through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3...proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and...activator by simply providing a new gRNA rather than engineering an entirely new protein. This makes it much cheaper...types of Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less about off...the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a member of the...develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.   ...Church GM (2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  9. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...briefing, to quick notes, to finances, to flights & ordering food - everything is covered.   So why are we ...folders in complex ways, there are solutions that bring more to the table in terms of data traceability ...researchers today generate vast amounts of digital data during their work. It is already becoming impossible to...to keep all their data in one place and avoid scattering intellectual property across various software...functionalities include comments, notifications, sharing files, assigning team members to certain tasks,...compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN...

  10. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...interested in measuring a population based on a fluorescent stain, you might be wondering which second ...types of scattering that occur when a cell is pulsed by the laser. Within each type of scattering, you also...to keep it short though, promise! FSC and SSC: scattering To digitally extract your cells based on their...cells stain positively for both markers. When comparing two markers simultaneously, we commonly describe...

  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...about the particular audience and venue before tailoring each presentation appropriately.  Find out who...many non-scientists be present?  Always ask the hiring manager or host for topic advice. An added bonus...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ... all graduate students and postdocs form peer mentoring groups to practice giving talks and getting feedback...always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...down it is a great segue to talk about how the sharing model works. See if you can elicit helpful comments...

  12. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...important information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,...readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular blog post...Grad School Advice Part 2: Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare...Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope you all have a great...

  13. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... what challenges remain in materials sharing, information sharing, and research reproducibility while ...service and support.  Alongside our material offerings, in 2022 we also rolled out an updated AAV Data...around the world can upload AAV validation data, sharing what works and, equally importantly, what doesn...commitment to supporting the research community through sharing science and distributing high-quality materials...

  14. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...social media, you could be sharing your research or finding opportunities to bring science to the community...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... the process down into steps and set aside time during the week to tackle each one.  Get the toughest ...

  15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent...Megamer service tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros...facilities for 13 projects, with most projects requiring only 40-60 zygotes to generate a correctly edited...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018... Mary Gearing...

  16. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...Westerns are divided into two categories, native and denaturing. In a native western, the protein’s secondary...is separated through a matrix by charge. In a denaturing western, the protein is denatured to its primary... purpose of this blog, we will be focusing on denaturing westerns.  Separating a protein mix by size Sodium...The wet transfer process is very effective in transferring a broad range of protein sizes but takes hours...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...loading control when assessing protein abundance or comparing samples across lanes.  For a typical loading control...

  17. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...year, graduate students and post-docs alike are wondering where the time has gone. Are we any closer to ...to graduating, publishing that key paper, or figuring out a career path? Many trainees are developing Individual...accomplishments, from learning new techniques to finally figuring out a tough experiment, sets a positive and encouraging...Good Manager Anyway Developing Transferable Skills During Science Training ... Mary Gearing...

  18. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    .... pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying sequence...affinity purification of protein complexes. John Pringle and Jürg Bähler have deposited a large collection...genome modification in yeast developed by Dr. Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2...McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine MS,... DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy...

  19. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...believing, as we do, that sharing speeds science.  This year, Chonnettia will be sharing more of her perspective...what we accomplished in 2023 and thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed... of innovation and impact made possible by the sharing of tools for research: discovered and developed...you a healthy, productive, and thriving 2024, mirroring our own aspirations for Addgene. On behalf of ...

  20. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types...science, we aim to accelerate the rate by which sharing speeds science.   Check out the precise neuronal...
Showing: 241 - 260 of 836 results