We narrowed to 517 results for: SET
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TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m.... not known why or how this happens. Therefore, I set out to study how protein turnover is regulated in...and specific antibody and the proper microscope setup we can observe the whole population of one particular...
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Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and in live zebrafish. For... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....tools and data that indicate the performance of (a set of) fluorescent proteins under specific conditions... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....). A control RFP expression plasmid. With this set up, all successfully transfected cells will express... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with an increased compatibility..., can only target certain sites, or make only a subset of base substitutions. The need for speed: base... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....submitting patent or grant applications; to informal settings like lab meetings, social media, coffee chats ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....compare to other antibody options out there, you’re set up for a successful next experiment! References... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....the basics of biosensors and how to use viruses to set up these systems. Components of a biosensor Some... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It...we would all like to steer clear of! However, a subset of viruses are commonly used in the lab as tools... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....stably integrate their genome into the host genome, setting up permanent residence. They primarily infect dividing... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem: There is no common method... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m.... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved...