We narrowed to 512 results for: SET
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TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating...
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Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....submitting patent or grant applications; to informal settings like lab meetings, social media, coffee chats ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....compare to other antibody options out there, you’re set up for a successful next experiment! References... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....the basics of biosensors and how to use viruses to set up these systems. Components of a biosensor Some... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It...we would all like to steer clear of! However, a subset of viruses are commonly used in the lab as tools... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....stably integrate their genome into the host genome, setting up permanent residence. They primarily infect dividing... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m.... not known why or how this happens. Therefore, I set out to study how protein turnover is regulated in...and specific antibody and the proper microscope setup we can observe the whole population of one particular... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and in live zebrafish. For... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....tools and data that indicate the performance of (a set of) fluorescent proteins under specific conditions... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....activation and base editing, whereas a different set of mutations (the “V3.1” version) works best for ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m.... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run. ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...