We narrowed to 852 results for: STI;

...chance as entrepreneurs. To make things more interesting, we are working in a new technical discipline... cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook from time to...worked weekends and evenings on the project, but still continued to generate new ideas as they popped into...doing makes sense. I can support that.” That was interesting. If people could be against GMOs as food but ... in the comments below. We’re happy to answer questions about this transition (so far) and what we’ve ...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...

...valuable laboratory tool are still an area of active research. New details are still being uncovered, and different...produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., ...et al., 2014). New discoveries on this topic are still being made! Conclusion Finally, we can summarize...their own advantages—and blind spots—to these questions. Each new structural insight also spurs the next...California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber...Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. Resources...

...The vast majority of bacteria are undomesticated which limits the tools scientists can use to study them...CRISPRi machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial...transfer Mobile-CRISPRi offers two advantages over existing bacterial CRISPRi methods. First, its modular ...Knockdown efficiency, as measured by knockdown of a constitutely expressed RFP reporter, was variable across ...Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown in presence of trimethoprim...Elizabeth A., and Pamela A. Silver. "Harnessing undomesticated life." Nature microbiology 4.2 (2019): 212....

...researchers have engineered alternative Cas9s binding distinct PAM sequences. Now, Hu et al., working in David...so far! Why do we need PAM flexibility? IDT estimates that there is an NGG PAM every 42 bases in the...edits or knock-ins. Editing efficiency decreases drastically once your cut site is more than 10 bp away from...frequency. Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter ...editing; for known promiscuous gRNAs, xCas9 3.7 still showed a 4.2-9.4-fold improvement in off-target:...these early data are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9...

...after graduate school, at least not while you’re still in the thick of it. About three or four years into...ways to get a taste of a variety of careers while still working towards your degree. For me, experimenting...The article submitted was hilarious, full of interesting information, and received a lot of attention ... said, “Boy, I love science,” and I realized, I still do too! Beyond the outreach experience I gained ...back into lab the next day and remembered why I still love science. Keep building on your time in graduate...look back at a positive, fulfilling experience. I still use my network and graduate school resources daily...

...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...The depositor name links to a page listing all of all this PI’s plasmids that are available through Addgene... plasmid page.  The paper link leads to a page listing all the plasmids from this paper that are available...Recipient Scientist Acknowledgement (RSA) asks requesting scientists to cite the depositing lab whenever...about how the vector was assembled, and enables requesting labs to utilize and modify the plasmid in their...will be to your colleagues. If you ever have any questions about our plasmid pages, please don't hesitate...

...homology-directed repair Homology-directed repair (HDR) stimulated by double strand breaks has been widely used ...application, and the efficacy varies widely — it still almost always results in higher ratios of desired...cost of improved efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for...obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The team also...additive improvements. These variants are worth testing if you want to make your prime editing as efficient...this tail is degraded, the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access...engineered virus-like particles. However, there’s still a long way to go before we see prime editors in ...

...Chapter 1 asks “What is science?” That’s a very big question. Zimmer, a Professor of Chemistry at Connecticut...chapters in this section describe the challenges of trusting science in a “fake news and post-truth era,” the...would be complete without the inclusion of some interesting case studies, and Zimmer provides several. In...throughout this chapter. Now, these observations and suggestions are not so much inaccurate as already somewhat...period of time. Though climate change certainly still is and will continue to be a “critical consequence...current period of rapid flux, the chapter might still feel slightly dated by next week.   A stepping stone...

...fifth repeat Nobel Prize winner in history. Interestingly, he also adds to the disproportionately large..., Provost partnered with colleagues at other institutions to form a support network called the MDH CUREs...MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and has helped provide... and results with peers and faculty at other institutions. Provost believes these networking experiences...Development. 24(5):127-130. Kimball, C., Powers, K., Dustin, J., Poirier, V., Pellettieri, J. 2020. The exon...purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent...

...correctly, there’s no point in crystallizing it or testing its function. Small ubiquitin-related modifier .... Arguably the simplest affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function...the tag size quickly – modifying the length a polyhistidine tract does not greatly alter the size of the...biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility...combinations, though frequently those combinations still include at least one element from the original TAP...purpose, and you don’t necessarily want them to stick around after that purpose has been served. At this...

...creative, usually saying that they’re not artists, or artistic, and therefore not creative. However, if we think...2019) If you’ve taken the time to do this and are still struggling with idea generation, try this thought...to the same problem. If your lab/company/team is still working towards a culture of encouraging dissent...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...easier to work with than nothing at all - they’re still ideas, after all, and that is the goal of this exercise... paint like Frida Kahlo or you’re stuck drawing stick figures, remember your training as a scientist has...

...BFP mutant (BFPH66) contains a histidine at amino acid 66. This histidine is encoded by a ‘CAC’ codon that...consists of a mutated mCherry and a downstream constitutively active eGFP. To create the inactive mCherry...T2A site and expressed in cells. mCherry is constitutively expressed to identify successfully transfected...C-to-T base editing event. This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66...markers, and other enzymes such as Cre-recombinase drastically expanding the base-editing reporter toolbox. ...

...we are investigating how the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...transient methods are usually the first choice for investigating gene function, as these experiments can be done...genes and speeding up research. Certainly, there is still more to transient leaf transformations, but we hope...

... spatiotemporal control over its expression. Constitutive or inducible Cre recombinases can be introduced...coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because CRISPRa can...long-term memory formation. The Bdnf gene possesses 9 distinct transcriptional start sites, all of which yield...singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with...notable exception) necessitates such molecular gymnastics for sensitive applications such as CRISPR screening...CRISPRa? Although cDNA overexpression approaches may still be preferable for individual gene activation in ...

...in what they can name a gene and many of the interesting ones come from the fly community. Not surprising...the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic Hedgehog, an important regulator of embryonic...got its name, we need to go back to 1980, when Christine Nusslein-Volhard and Eric Wiechaus first identified...cytokinesis. Why name this protein after a vegetable substitute for spaghetti? I actually couldn’t find the specific...failure of primary spermatocytes to enter meiosis. Testis of cookie monster mutant flies are filled with ...1189. PubMed PMID:1905980. Nüsslein-Volhard, Christiane, and Eric Wieschaus. "Mutations affecting segment...

..., a pooled library has hundreds to millions of distinct plasmids, all of which need to be represented ...The size and composition of your library can drastically impact what conditions it needs to properly amplify...pre-made libraries aren’t quite plug-and-play: you’ll still need to titer and optimize before you can transduce...your research. And, of course, if you have any questions, don’t hesitate to email us at [email protected], this is a useful place to ask experimental questions.  Practical Considerations for Using Pooled Lentiviral...lentiviral CRISPR libraries. Protocols from the Broad Institute- A variety of protocols and guides regarding pooled...

... neuronal populations in the brain. The Allen Institute and the Janelia Research Campus in the USA have...classic probes. To overcome the limitations of existing FP and epitope tags, researchers in Loren Looger...set-up. In contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific...smFP_FLAG than GFP.   3. Enhanced ability to distinguish weakly expressed and/or similar proteins Through...proteins than standard tags and can be used to distinguish highly similar proteins. For example, N-cadherin...terms of sequence and thus very difficult to distinguish using standard antibodies. By fusing N-cadherin...

... proactively address questions about your application. These could be logistical, like briefly explaining...and it does take a bit of time to craft one. We still think they're useful, so we've updated our cover...and used a ddPCR assay to answer an experimental question. Now, the hiring manager knows that you can design...most important skills for the position]. I am enthusiastic about [something that you love and is important...any unusual circumstances or proactively answer questions the hiring manager may have. The closing paragraph...

...echoed in the other testimonies we gathered. The program definitely caused a positive stir and was recognized...invaluable to me as a bench scientist working at an institution where training opportunities weren’t always available...were connected to that Tweet Up in 2013. These existing connections brought in folks I didn’t yet know....  They liked us. They really liked us.  The testimonials from participants in Binning Singletons at ASM...Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith...

...by enhanced green fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated ...mediated EGFP expression cassette reconstitution. When the target sequence was digested by sgRNA guided Cas9 ...SSA, single strand annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP...details on this plasmid, see Mashiko et al., 2013). Testing the efficacy of your gRNAs is then a simple matter...of gene modification in vivo. Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene...require you to house mice that may have no mutation, costing money and space. As a rule, screen all of your ...