We narrowed to 501 results for: SUB

...transcription to convert amplified DNA to RNA for subsequent detection by Cas13. This amplification step in...trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded at a rate of ~1,250 cuts per second...

...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...behavior. Signs of a mycoplasma contamination can be subtle and may include reduced saturation density, decreased...

...eukaryotic) proteins localize to chemically distinct subcellular environments, including the organelles that compose...focused on optimizing fluorescent proteins for subcellular environment. Currently, she is a SPIRE postdoctoral...

...should not effected by claims that ‘facts’ are subjective or alternative. Inclusion of data informing...informing policy specifically related to the following subjects: Climate change Vaccines GMOs Transgender issues...

...N-terminus, but this hardly reduces its size. The substantial size of GFP may interfere with the activity or...generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal ...

...are identified by PCR. Then, if necessary for subsequent experiments, the floxed neoR gene is removed ..., and are therefore able to infect only a small subset of the cells used in a given experiment. For this...

...are already done. Intracellular labeling can be subdivided into two categories, depending on the location...DNA during DNA synthesis and then diluted with subsequent rounds of DNA synthesis (cell divisions).   ...

...deposited several constructs fused with popular subcellular markers like Lifeact, Sec61β, H2B, and more. ...preserved. The team used TimeVault to show that a subpopulation of PC9 lung cancer cells had a distinct transcriptome...

...evolution, introducing mutations to the deaminase subunit to produce a series of ABE8s that were faster, ...., Spinner, A. D., Lazzarotto, C. R., Li, Y., Matsubara, A., Urbina, E. O., Dahl, G. A., Correia, B. E...

...prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic archea: these organisms lacked ...phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express the completely...

...resulted in appropriately localized dystrophin. In a subsequent study, they used CRISPR with two gRNAs to delete... mRNA is high, likely because this mRNA is not subject to nonsense-mediated decay. Although Nelson et ...

...excision results in one of three recombinations, and subsequent recombinations cannot occur. While the Brainbow...recombinase-based fluorescence labeling technique to subdivide neural expression patterns. Hampel S, Chung P,...

...only includes general lab strains designed for subcloning or protein expression. If you were to include...exogenous proline source for growth rpsL Mutation in subunit S12 of 30S ribosome Confers resistance to streptomycin...

... don’t fit in either category, but those are a subject for a future post! How are repressible promoters...the Q system is also functional in C. elegans. Subsequent work by the Luo and Potter labs has refined the...

...setup. A light source delivers light pulses to the subject/cells expressing the optogenetic tool causing a...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ...

...surface proteins with a membrane-impermeable biotin substrate (Figure 1). Biotinylated proteins were isolated...soma-targeted variant SomaFRCaMPi also performed substantially better than other soma-localized red GECIs, ...

...and others, we have updated our kit process substantially and have plenty of ideas for further improvements...fill and what their responsibilities are. That’s a subtle but important distinction. If I invite someone ...

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...to develop “AAV 3.0”, vectors that will have substantially improved performance profiles and should be ...

... of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal typically...burnout The suggestions in this blog add up to a substantial amount of time- most of your waking hours. This...

...design gRNAs. A more extended discussion of these subjects can be found in two recent review articles (Doench...involves the need for single cell cloning and subsequent screening for successful edits. This is a very...