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Showing: 241 - 260 of 489 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...are already done. Intracellular labeling can be subdivided into two categories, depending on the location...DNA during DNA synthesis and then diluted with subsequent rounds of DNA synthesis (cell divisions).   ...

  2. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...prokaryotic lineages revealed a curious anomaly amongst a subset of methanogenic archea: these organisms lacked ...phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express the completely...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...resulted in appropriately localized dystrophin. In a subsequent study, they used CRISPR with two gRNAs to delete... mRNA is high, likely because this mRNA is not subject to nonsense-mediated decay. Although Nelson et ...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...only includes general lab strains designed for subcloning or protein expression. If you were to include...exogenous proline source for growth rpsL Mutation in subunit S12 of 30S ribosome Confers resistance to streptomycin...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...Liver AAV8 Sands, 2011 Lung pUCmini-iCAP-AAV9.452sub.LUNG1 Goertsen et al., 2022 Pancreas AAV6, AAV8...designs and administration doses. Don't forget to submit your validation data after your experiment! Thanks...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Datatable. A more extended discussion of these subjects can be found in two recent review articles (Doench...involves the need for single cell cloning and subsequent screening for successful edits. This is a very...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...setup. A light source delivers light pulses to the subject/cells expressing the optogenetic tool causing a...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... don’t fit in either category, but those are a subject for a future post! How are repressible promoters...the Q system is also functional in C. elegans. Subsequent work by the Luo and Potter labs has refined the...

  10. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...to develop “AAV 3.0”, vectors that will have substantially improved performance profiles and should be ...

  11. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...and others, we have updated our kit process substantially and have plenty of ideas for further improvements...fill and what their responsibilities are. That’s a subtle but important distinction. If I invite someone ...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...imparts TALENs more specificity and a broader substrate preference than the ZFNs.   Genetic Engineering... (1944) STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES....

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal typically...burnout The suggestions in this blog add up to a substantial amount of time- most of your waking hours. This...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...to uracil without cutting DNA. Uracil is then subsequently converted to thymine through DNA replication...produces stable edits. However, this system is also subject to off-target editing based on Cas9 off-target ...

  16. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments, like ...

  17. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful ...

  18. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ... you're designing your next virus experiment. Subscribe to Viral Vector Blog Posts from Addgene Need ...

  19. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...every quick tip video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols...
Showing: 241 - 260 of 489 results