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...serotype of our AAV preparations. We recently published a description of the VGS process and use cases...doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer A, Mevel M, ...

...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

...using the power of light. In a recent article published in Nature Methods, Boyden and his team describe...can figure out what they are necessary for. We published our first paper on optogenetic tools for neuroscience...

...analysis script should return the same output: a spreadsheet of read counts. You can use these counts to confirm...and maintenance of good representation. Addgene wishes you luck in your future library use and screening...

...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is exceptionally ...

...Additionally, proper citation of these Addgene IDs in published work allows readers to quickly track down the ...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org...

...made to moderate and cross-check all data with published literature as soon as possible after submission... queries.  Looking for a bright, monomeric FP published in the last few years with an emission peak around...

...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ...

...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...tracking their fulfillment manually in a shared spreadsheet and it takes time away from our research. For...

...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered...

...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

...Scientists Using Social Media" tip sheet from Science Media Savvy This tip sheet provides advice for tweeting...

...why the results you've measured should change established theory. So, what can you do to ensure that your...available. But think of this as a jumpstart or a refresher for your skillset rather than a permanent fix....

...homologue) or extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes...Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683...

...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...

...self-assembling protein (SAP) such as a bacteriophage shell protein or a bacterial microcompartment protein....expressing the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

... kits to make their life easier. Recently, we published a blog on DNA purification without a kit that ...molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol on how to integrate RNAlater...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...the basic assay remains widely used today.  The sheer abundance of these critical research tools is a ...

...across the globe. This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli... Alyssa Shepard...